3-Bromocinnamic acid
Molecular weight: 227.05
EINECSNO.:251-267-7
Melting point: 177-179 °C
Appearance: Colorless crystal
Vapor pressure: 0.0±0.8 mmHg at 25°C
Refractive index: 1.648
Storage: Shading, confined preservation, dry, ensure good ventilation or exhaust in the workshop
Stability: Stable under normal temperature and pressure
Key words: 3-Bromocinnamic acid, spices, essence, Synthetic essence,
General description
3-Bromocinnamic acid is the metabolite of cinromide (3-bromo-N-ethylcinnamamide) and can be analysed in plasma by high-performance liquid chromatographic method.
Simultaneous determination of the anticonvulsants, cinromide (3-bromo-n-ethylcinnamamide), 3-bromocinnamamide, and carbamazepine in plasma by high-performance liquid chromatography.
3-Bromocinnamic acid is used as organic synthesis of raw materials, fine chemicals, pharmaceutical intermediates.
Abstract
A high-performance liquid chromatographic method is described for monitoring plasma concentrations of cinromide (3-bromo-N-ethylcinnamamide) and its de-ethylated metabolite. Carbamazepine levels can be easily measured by the same technique. The N-isopropyl analogue of cinromide is used as internal standard, and all compounds are easily separated on a reversed-phase column operated at 55 degrees with a small-diameter pre-column maintained at the same temperature. The extraction is rapid and generally applicable to plasma and urine samples that are to be analyzed by reversed-phase chromatography. Short- and long-term reproducibility studies show less than 4% relative standard deviation for replicate determinations for all drugs. Limits of quantitation are 10-20 ng/ml with an internal standard concentration of 3 micrograms/ml. Another metabolite of cinromide, 3-bromocinnamic acid, which may have some anticonvulsant effect, can be analyzed simultaneously by buffering the mobile phase and adding an ion-pairing reagent.Storage:Pay attention to the source of fire, especially to avoid sunlight, and store in a cool place.
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