Recombinant Carboxypeptidase B（with His Tag）
CAS: 9025-24-5
Recombinant Carboxypeptidase B（with His Tag） is a lyophilized powder that is isolated and purified from recombinant Pichia pastoris and then freeze-dried, and has the same enzyme activity and specificity as natural carboxypeptidase a and b.
Recombinant Carboxypeptidase B （with His Tag）Specifications
|
Product Name |
Recombinant Lysyl Endopeptidase (Biochemical Grade) |
|
Appearances |
White or off-white powder |
|
Specific Activity |
≥80U/mg pro |
|
MW |
36kD±3.6kD |
|
Purity |
≥95%（SDS-PAGE） |
|
Protein Content |
20%-60% |
|
Residue DNA |
≤100ng/mg pro |
|
Residue HCP |
≤100ng/mg pro |
|
Optimum pH |
|
|
Solubility |
Soluble in buffer |
|
Storage Conditions |
-15°C below, protected from light，sealed. |
Carboxypeptidase B Application
Production of various proteins or polypeptides, such as insulin and analogs, cosmetic peptides, antimicrobial peptides, food flavor peptides, and the like.
Quality testing of antibodies.
Determination of the C-terminal amino acid of a protein
Carboxypeptidase B Recommended Method
Protein preparation: The protein was dissolved in a solution of 25 mM Tris-HCl, pH 7.6.
Enzyme preparation: The desired enzyme powder was weighed and dissolved in 25 mM Tris-HCl (pH 7.6) to give an enzyme solution concentration of 1~10 mg/ml.
Reaction recommended enzyme amount: target protein (mass ratio) = 1:50 ~ 1:1000, optimum pH is 7.0~9.0.
Recombinant Carboxypeptidase-BTransport and Storage Stability
Transportation stability: It is transported in an ice pack to keep it active.
Storage stability: The lyophilized powder can be stored for at least 24 months at -15°C below.
25 mM Tris-HCl (pH 7.6) was dissolved and stored at -15°C below, and repeatedly frozen and thawed 10 times without loss of activity.
It is recommended not to exceed 7 days after storage at 2~8°C, and no more than 30 days for -15°C below.
Notice and Disclaimer
Activity is competitively inhibited by arginine and lysine, and metal ion chelating agents such as EDTA also inhibit enzyme activity.
The product is hygroscopic and should be taken after equilibration at room temperature.
The product is for research use only and should not be used for diagnosis or treatment of animals or humans.
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