High purity Phospho-L-Tyrosine Disodium Salt
Phospho-L-Tyrosine Disodium Salt in Biochemical Research: Standards and Assay Tools
Phosphorylation Detection Standards: Used as a reference standard in Western blotting, ELISA, HPLC, and mass spectrometry (MS) to quantify phosphotyrosine residues in proteins. For example, it serves as a calibration curve standard in phospho-specific antibody validation, enabling accurate quantification of tyrosine phosphorylation levels in cell lysates or purified proteins.
Phosphatase Activity Assays: Acts as a specific substrate for tyrosine phosphatases (e.g., PTP1B, SHP-2) to evaluate phosphatase catalytic activity, kinetic parameters (Km, Vmax), and inhibitor efficacy. The dephosphorylation product (L-tyrosine) is detected via UV spectroscopy or fluorescence, facilitating high-throughput screening of phosphatase modulators.
Signal Transduction Pathway Studies: Added to cell culture systems to simulate tyrosine phosphorylation events, investigating downstream signaling cascades (e.g., PI3K/Akt, MAPK pathways). It helps verify whether target proteins (e.g., EGFR, JAK2) are regulated by tyrosine phosphorylation.
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