Human Amyloid beta peptide 1-42(Aβ1-42)ELISA Kit

Thiskitusesenzyme-linkedimmunesorbentassay(ELISA)basedontheBiotindoubleantibodysandwichtechnologytoassaytheHumanAmyloidbetapeptide1-42(Aβ1-42).AddAmyloidbetapeptide1-42(Aβ1-42)tothewells,whicharepre-coatedwithAmyloidbetapeptide1-42(Aβ1-42)monoclonalantibodyandthenincubate.Afterthat,addantiAβ1-42antibodieslabeledwithbiotintounitewithstreptavidin-HRP,whichformsimmunecomplex.Removeunboundenzymesafterincubationandwashing.AddsubstrateAandB.Thenthesolutionwillturnblueandchangeintoyellowwiththeeffectofacid.TheshadesofsolutionandtheconcentrationofHumanAmyloidbetapeptide1-42(Aβ1-42)arepositivelycorrelated.

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