TES BUFFER CAS NO.7365-44-8
The name of TES is n-trimethylol-methyl-2 amino-ethanesulfonic acid. TES powder can not only be prepared into biological buffer, but also is a very important chemical raw material. It is widely used in biochemical research to regulate the pH of biochemical reactions. It is generally used as a dissolving agent or solution pH maintainer.
High quality TES(CAS No. 7365 44 8) is a white crystalline powder. It is quite similar to Tris-HCl powder. When the pH value of biochemical detection solution is 6.8-8.2, the buffering capacity of TES buffer can play the greatest role. TES buffer is an electrically neutral compound. At the same concentration, the force between TEs ions is greater than that between Tris ions. When detecting metal ions, TES buffer will not react with metal ions to produce other metal compounds. Therefore, when TES is used as the buffer solution of cell culture medium, it can provide positive divalent metal ions for the culture medium.
TES Buffer (CAS No. 7365-44-8) Detailed Introduction
The solubility of TES in water is very high. When 5g tes is added to 100ml water, it can be completely dissolved and reach the saturated state. After dissolution, it is transparent. The solubility of TES will not change greatly with the change of temperature. As a biochemical buffer, TES buffer does not participate in chemical reactions, so it will not affect the research results. Desheng is a professional source manufacturer of TES. Our company can ensure that the TES crystal powder produced is pure white with a purity of 99%. With mature production technology and perfect equipment, we can ensure that the daily output of TEs can reach 2 tons per day, and fully meet the production delivery date under the condition of ensuring the quality.
TES Buffer (CAS No. 7365-44-8) Advantages
TES is a non-toxic buffer;
High solubility in solution, so as not to waste reagent;
In the pH range of 6.5-8.2, it has good stability and can keep the pH value of the solution in a stable state for a long time;
The chemical property is not active and does not interfere with the test results due to chemical reaction with enzymes and metal ions;
Little absorption of visible light or ultraviolet light.
Superb purification technology can ensure that the purity is higher than 99% and almost free of other impurities;
The experienced production team and quality assurance personnel shall strictly control the quality of TES;
Professional R & D team can provide TES buffer samples for testing and detection according to customer requirements.
TES Buffer (CAS No. 7365-44-8) Applications
In the process of oxidation of succinic acid (succinic acid) to carbon dioxide, TES is more suitable than other buffers to maintain the pH of the solution;
TES is needed as a buffer medium to ensure the accuracy of the test in the refrigeration and transportation of sperm yolk storage solution;
In protein purification, separation and determination, TES buffer is used to maintain the pH value required by the test;
Like most amine buffers, TEs can form complexes with DNA, so it can be used as cell washing buffer;
TES, 4-hydroxyethyl piperazine ethanesulfonic acid and trimethylolaminomethane can inhibit the activity of the enzyme, but the influence of TEs on the enzyme is between the other two;
TES can affect the expression of messenger RNA and embryonic development when measured in dioctenic acid (BCA);
In personal care and cosmetic applications, TEs can be used to protect the active ingredients in the formula from degradation;
TES solvent can be used to separate hemoglobin and protect the stability of hemoglobin after freeze-drying;
Finally, TES buffer can be used as cathode solution in 2-D gel isoelectric focusing electrophoresis (IEF).
TES Buffer (CAS No. 7365-44-8) Precautions
When detecting positive divalent copper ions, TES cannot be used because they will form a complex;
The buffer capacity is affected by temperature, and the configuration temperature should be controlled at room temperature;
TEs can affect the expression of messenger RNA and embryonic development;
There is little complexation between TEs and metal, but once complexation occurs, interference may occur;
Usually put it in a cool, dry and ventilated place to prevent tes crystal powder from getting wet;
After the solution is prepared, it should be prepared and used first. It is easy to produce impurities after long-term storage.
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