CapitalBio® Microarray Hybridization Station

CapitalBio® Microarray Hybridization Station

The CapitalBio® BioMixerTMII is a microarray hybridization station that uses continuous 3-D motion to help the aqueous hybridization mixture spread evenly across the microarray surface in a temperature-controlled environment. This uniformly exposes each sample spot to the reaction reagent solution, resulting in increased reaction signal intensity and signal consistency. It is simple to use, with up to 6 or 12 microarray slides hybridized simultaneously. The microarray platform comes with three interchangeable adapters for hybridization, washing, and mixing biochemical reagents in tubes.

Features of CapitalBio® Microarray Hybridization Station

When combined with CapitalBio's specially designed hybridization cassettes, uniform temperature and humidity are achieved.

Unique 3-D motion guarantees the hybridization signal is homogenous over the whole microarray surface.

Eliminates edge effects. Enhanced results evidenced in recent independent reports on microarray quality and performance, such as Patterson, T., et al., (2006) Nature Biotechnology 24: 1140-1150 and Shi, L., et al., (2006) Nature Biotechnology, 24: 1151-1161.

LCD control panel.

Suitable for microarrayhybridizations and immunoreactions on 25 mm × 75 mm slides or in tubes using different adaptors.

What Is The Difference Between Microarray Hybridization And Northern Hybridization?

Northern hybridization, also known as Northern blotting, is a technique used to detect specific RNA molecules within a mixture. In this process, a single DNA molecule is labeled and hybridized with an unlabeled mixture of RNAs. This allows for the detection and quantification of specific RNA molecules, making it a targeted approach.

Microarray hybridization is a high-throughput method that allows for the simultaneous analysis of thousands of genes. In this technique, a mixture of target RNA, which can consist of many species of molecules, is labeled. This labeled mixture is then hybridized into a solid surface containing thousands of immobilized probes, each corresponding to a specific gene. This allows for the simultaneous detection and quantification of many different RNA molecules, providing a comprehensive analysis of gene expression.

The main difference between the two techniques lies in their scale and specificity. While Northern hybridization focuses on a single RNA molecule, microarray hybridization allows for a broader, more comprehensive analysis. Both techniques have their advantages and are used in different contexts depending on the research needs.

What Is The Principle Of Microarray Hybridization DNA Chip Technology?

Microarray hybridization is a technique based on the principle of complementary base pairing. In this process, DNA strands from a sample are labeled and applied to a DNA microarray, where they seek out and bind to their complementary sequences. These sequences are immobilized onto specific spots on the microarray. The binding process, known as hybridization, is facilitated by the microarray hybridization station, which maintains optimal conditions for hybridization to occur.

The use of a microarray hybridization station ensures a high degree of accuracy and efficiency in the hybridization process. This, in turn, allows for the simultaneous analysis of thousands of genes, providing comprehensive insights into gene expression and genetic variation. CapitalBio Technology utilizes this technology to deliver precise and reliable genomic analyses.

Specification of CapitalBio® Microarray Hybridization Station

Features	Parameters
Capacity	6 or 12 slides/15 ml tubes/50 ml tubes
Time Range	1min ~ 23hr59min
Temperature Range	30 ~65°c
Temperature Accuracy	±0.5°c
Rotating Speed	5 ~ 30 rpm (adjustable)
Voltage	100 ~240 V
Power	1,000 Watts
Size (W x Dx H)	405mm x 516mm x 486mm (15.9 in x 20.3 in ×19.1 in)
Weight	36 kg (79 lb)

How Is Hybridization Used In Microarray?

Microarray hybridization is a process where two samples of cDNA, labeled with different fluorescent dyes, are allowed to bind to a microarray slide.

The microarray slide contains a grid of DNA probes, each representing a different gene.

The hybridization reaction occurs when these cDNA molecules bind or ‘hybridize’ to the complementary DNA probes on the slide. This binding is highly specific.

After the hybridization process, the microarray slide is placed in a microarray hybridization station provided by CapitalBio Technology. This device maintains the optimal conditions for hybridization, ensuring that the process is efficient and accurate.

The microarray hybridization station is crucial in controlling the environment during the hybridization process, which can significantly impact the results.

The microarray is then scanned to measure the fluorescence of each spot on the slide. The intensity of the fluorescence indicates the expression level of each gene.

By comparing the fluorescence of the two different dyes, researchers can determine changes in gene expression between the two samples.

CapitalBio Technology’s microarray hybridization station is a key tool in this process, providing reliable and efficient solutions for researchers worldwide.

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