Fluorescence Filters
Fluorescent filterare a class of filters classified by application type.
Fluorescence microscopy filtersis a fluorescence imaging filter for biomedical and life science instruments, the key components, the main role is in the biomedical fluorescence analysis system for the separation and selection of substances in the excitation and emission fluorescence Of the spectral characteristics of the band. It is usually required that the filter cut-off depth be greater than OD5 (optical density, OD = -lgT). The core requirements for filters used in fluorescence detection systems are high cut-off steepness, high transmittance, high positioning accuracy, high cut-off depth, and excellent environmental stability.
Fluorescent filter is a combination of three, three are excitation filters, emission filters and dichroic filters.
Excitation Filter (Exciter Filter, Excitation Filter, Excitation Filter): In the fluorescence microscope, only the excitation wavelength of the filter can pass through the fluorescence. In the past, a short-pass filter was used, and now a band-pass filter is basically used. The housing is marked with arrows indicating the direction of propagation of the recommended light.
Emission Filter (Emitter Filter, Emitter): Select and transmit the fluorescence emitted by the sample, the other range of light cut-off. The wavelength of the emitted light is longer than the wavelength of the excitation light (closer to red). A band-pass filter or a long-wave-pass filter may be selected as the emission filter. The housing is marked with arrows indicating the direction of propagation of the recommended light.
Dichroic Mirror (Dichromic Beamsplitter, Dichromatic Beamsplitter): also known as dichroic mirrors or dichroic mirrors. And placed at an angle of 45 ° to the optical path of the microscope. This filter reflects one color of light (excitation light) and transmits another color of light (emitted light), the reflectivity of the excitation light is greater than 90% and the transmittance of the emitted light is greater than 90%. The impervious portion of the spectrum is reflected rather than absorbed. Filter in the transmitted light and reflected light color complement each other, and thus also known as dichroic filters.
Our fluorescence filterare designed for fluorescence imaging applications, with durability in mind and high-performance optical specifications in manufacturing. The filter substrate is made of quartz, which can achieve 1/10 lambda surface accuracy, while the thermal expansion coefficient of quartz is relatively small, can obtain higher image quality.
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