Inorganic Pyrophosphatase

• 

Inorganic Pyrophosphatase is a highly thermostable enzyme derived from extremophilic bacteria. It efficiently catalyzes the hydrolysis of inorganic pyrophosphate (PPi) into two orthophosphates (Pi), relieving the inhibitory effect of PPi accumulation during nucleic acid synthesis. It is widely used in molecular biology experiments and industrial production.

PPase is purified from an E. coli strain carrying an inorganic pyrophosphatase gene cloned from Thermococcus thermophilus.

Inorganic pyrophosphatase (PPase) catalyzes the hydrolysis of inorganic pyrophosphate to produce orthophosphate.

Applicable Scenarios:

PCR/qPCR amplification, NGS sequencing, in vitro transcription (IVT) mRNA synthesis, enzymatic industrial production, DNA/RNA-related reagent development

Product Advantages:

Excellent thermostability: retains 100% activity after treatment at 100°C for 4 hours.The enzyme has a wide range of applications, including optimization of RNA transcription - increasing RNA yield in in vitro transcription reactions; optimization of PCR reactions - increasing DNA yield; removal of PPi contaminants from reagents used for SNP genotyping using the pyrophosphate assay; promoting the synthesis of proteins, RNA, and DNA; and catalyzing reactions related to PPi + H2O → 2Pi. The enzyme is highly active and thermally stable, insensitive to temperature and can be accelerated by increasing the temperature to accelerate the kinetic reaction, and remains active after repeated freezing and thawing.

Extreme heat resistance: Extremely high thermal stability, 100°C treatment for 4 hours still retains 100% activity

Significantly increases yield: Efficient hydrolysis of PPi, inhibits inhibitors, synthesis efficiency increased by 20–50%

Reaction system purity:Adaptable to various extreme reaction environments, no nuclease

contamination interference

Other supplier products

	SUMO Protease	SUMO Protease is a highly specific recombinant protease used for precise removal of SUMO tags from fusion proteins. After cleavage, no extra amino ...
	Acetylesterase	Acetylesterases are a class of enzymes that are specialized for breaking down acetylester bonds in substrates. These enzymes exist widely in nature...
	Inorganic Pyrophosphatase	Inorganic Pyrophosphatase is a highly thermostable enzyme derived from extremophilic bacteria. It efficiently catalyzes the hydrolysis of inorganic...
	Gas Well Guar Gum Enzyme Breaker	Fusozyme® Gas Well Guar Gum Enzyme Breaker is a high-efficiency enzyme-based unblocking agent developed specifically to address formation resid...
	T7 RNA Polymerase	T7 RNA Polymerase is a highly specific RNA polymerase derived from bacteriophage T7. It efficiently catalyzes the transcription of DNA templates in...

Same products

	Asparaginase	Seller: BIT Bio-harbor Company	Asparaginase (ASN) is an amide hydrolase that specifically catalyzes the hydrolysis of asparagine...
	Acetylesterase	Seller: BIT Bio-harbor Company	Acetylesterases are a class of enzymes that are specialized for breaking down acetylester bonds i...
	β-N-Acetylglucosaminidase	Seller: BIT Bio-harbor Company	β-N-acetylglucosaminase is a lysosomal enzyme used to hydrolyze n-acetyl-β-D-glucosamin...
	Chitinase	Seller: BIT Bio-harbor Company	Chitinase is an efficient extracellular enzyme complex for chitin degradation, derived from Serra...
	TEV Protease	Seller: BIT Bio-harbor Company	TEV Protease (Tobacco Etch Virus protease) is a commonly used protein purification tool enzyme wi...