Inorganic Pyrophosphatase
Inorganic Pyrophosphatase is a highly thermostable enzyme derived from extremophilic bacteria. It efficiently catalyzes the hydrolysis of inorganic pyrophosphate (PPi) into two orthophosphates (Pi), relieving the inhibitory effect of PPi accumulation during nucleic acid synthesis. It is widely used in molecular biology experiments and industrial production.
PPase is purified from an E. coli strain carrying an inorganic pyrophosphatase gene cloned from Thermococcus thermophilus.
Inorganic pyrophosphatase (PPase) catalyzes the hydrolysis of inorganic pyrophosphate to produce orthophosphate.
Applicable Scenarios:
PCR/qPCR amplification, NGS sequencing, in vitro transcription (IVT) mRNA synthesis, enzymatic industrial production, DNA/RNA-related reagent development
Product Advantages:
Excellent thermostability: retains 100% activity after treatment at 100°C for 4 hours.The enzyme has a wide range of applications, including optimization of RNA transcription - increasing RNA yield in in vitro transcription reactions; optimization of PCR reactions - increasing DNA yield; removal of PPi contaminants from reagents used for SNP genotyping using the pyrophosphate assay; promoting the synthesis of proteins, RNA, and DNA; and catalyzing reactions related to PPi + H2O → 2Pi. The enzyme is highly active and thermally stable, insensitive to temperature and can be accelerated by increasing the temperature to accelerate the kinetic reaction, and remains active after repeated freezing and thawing.
Extreme heat resistance: Extremely high thermal stability, 100°C treatment for 4 hours still retains 100% activity
Significantly increases yield: Efficient hydrolysis of PPi, inhibits inhibitors, synthesis efficiency increased by 20–50%
Reaction system purity:Adaptable to various extreme reaction environments, no nuclease
contamination interference
Send product request
Other supplier products
 |
Conventional Fracturing Guar Gum Enzyme Breaker | Fusozyme® Enzyme Breaker for conventional guar gum fracturing systems is specially designed for conventional wells and certain low-temperature ... |
 |
Shale Oil Guar Gum System Enzyme Breaker | This enzyme product is developed based on synthetic biology technology with AI-assisted structural redesign. By optimizing mannosidase/mannanase ge... |
 |
Enzyme Breaker for Conventional Slickwater Fracturing | Fusozyme®Enzyme breaker for conventional slickwater fracturing is an enzymatic depolymerizer specifically designed for slickwater and polyacryl... |
 |
Acetylesterase | Acetylesterases are a class of enzymes that are specialized for breaking down acetylester bonds in substrates. These enzymes exist widely in nature... |
 |
Enzyme Breaker for Coal Bed Methane Slickwaterr | Fusozyme® Enzyme Breakertargets PAM fracturing fluid for coalbed methane. It breaks gel thoroughly, mitigates formation damage and boosts recov... |
Same products
 |
Asparaginase | Seller: BIT Bio-harbor Company | Asparaginase (ASN) is an amide hydrolase that specifically catalyzes the hydrolysis of asparagine... |
|
|
Acetylesterase | Seller: BIT Bio-harbor Company | Acetylesterases are a class of enzymes that are specialized for breaking down acetylester bonds i... |
 |
β-N-Acetylglucosaminidase | Seller: BIT Bio-harbor Company | β-N-acetylglucosaminase is a lysosomal enzyme used to hydrolyze n-acetyl-β-D-glucosamin... |
 |
Chitinase | Seller: BIT Bio-harbor Company | Chitinase is an efficient extracellular enzyme complex for chitin degradation, derived from Serra... |
 |
TEV Protease | Seller: BIT Bio-harbor Company | TEV Protease (Tobacco Etch Virus protease) is a commonly used protein purification tool enzyme wi... |
