TEV Protease

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TEV Protease (Tobacco Etch Virus protease) is a commonly used protein purification tool enzyme widely applied in biological research. It can specifically recognize and cleave fusion proteins containing the ENLYFQ↓S/G sequence, thereby removing the tag protein from fusion proteins to obtain pure target proteins. It is ideal for recombinant protein expression and purification, and widely applied in structural biology and protein-related research.

Applicable Scenarios:

Protein engineering and functional validation in basic biological research; tag removal and intermediate purification in antibody drug and vaccine development; customized protein cleavage

Product Advantages:

Compatible with a wide range of pH values and buffers; high cleavage efficiency and specificity.

1. Extreme Condition Stability: Tolerates high concentrations of imidazole (e.g., Ni column elution conditions) and various salts (e.g., NaCl, KCl), eliminating the need for additional purification steps and enabling compatibility with complex buffer systems.
2. Broad Temperature Activity Range: Maintains high catalytic efficiency across 4–37°C, suitable for diverse experimental scenarios (e.g., gentle cleavage at low temperatures or rapid reactions at ambient conditions), ensuring operational flexibility.
3. Precise and Efficient Cleavage: Exclusively targets the ENLYFQG site without nonspecific side reactions, preserving the integrity of the target protein.

Extremely high recognition specificity: Only recognizes and cleaves ENLYFQ↓S/G sequence, no non-specific cleavage

Removal process simple: Molecular weight approximately 27–28.3 kDa, easily removed by affinity chromatography

Mild reaction conditions: Work temperature range wide (4°C–30°C), protects sensitive target protein activity

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