Hydrophobic Interaction Chromatography
In the downstream separation and purification process of biopharmaceuticals, chromatography is one of the indispensable separation methods. BioLink provides customers with 5 different chromatography solutions, including gel filtration chromatography, ion exchange chromatography, affinity chromatography, hydrophobic exchange chromatographyand multimodal chromatography. In addition, BioLink also produces chromatography columns and prepacked columns from laboratory to industrial grade. We focus on providing customers with high-quality products, professional solutions and comprehensive after-sales services.
How To Select The Appropriate Chromatography Resin For My Own Process?
Since the hydrophobic characteristics of the target product are difficult to obtain directly, when designing a hydrophobic chromatography process, pre-experiments should be carried out with fillers containing different hydrophobic ligands. The hydrophobicity of the hydrophobic ligand butyl-octyl-phenyl increases sequentially, and the hydrophobicity of the target product can be predicted through the combined adsorption test with different hydrophobic fillers. After understanding the hydrophobicity of the target product, pretreatment of the target product should be carried out. Generally speaking, the target product will be combined with a hydrophobic filler in a high-salt system and eluted under a low-salt condition. Therefore, it is necessary to optimize the salt concentration during sample loading(ammonium sulfate is usually selected as the pretreatment salt for sample loading). In addition, after preliminarily determining the type of ligand and the salt concentration required for sample loading, the gradient elution from high salt to low salt can be used for filler screening and process optimization. The follow-up can be further optimized through experimental design.
Hydrophobic Chromatography Packing Suitable Samples
Hydrophobic chromatography is a chromatographic mode for purification based on the difference in hydrophobicity between the target product and impurities. Generally speaking, hydrophobic chromatography is mainly used for the removal of high molecular weight polymers.
Send product request
Other supplier products
BioHub MIX Single-use Desktop Mixing System | The small size design is equipped with a unique paddle to meet the mixing of small volume of material and liquid. Please be advised that this pr... | |
BioHub SC Sealer | BioHub® Sealer can be used to disconnect disposable components used in biopharmaceutical manufacturing processes, maintaining product sterility... | |
Cell Culture Bag | CytoLinXWBseriessingle-usecellculturebagsaresuitableforwavecellculturesystems,designedforcommoncellcultureapplicationsinbiopharmaceuticals.Cellcult... | |
Single-Use Bioprocess Bags and Bottles | Bio-Link offers a wide range of single-use bioprocess containers covering liquid storage, transport, sampling, mixing, cryopreservation, and final ... | |
BioHub 3D Disposable Bioprocess Bags | BioHub 3D Disposable Bioprocess Bags BioHub® 3D single-use bioprocessing bags are designed for bioprocess needs. This 3D biocontaineris suitab... |
Same products
Railway stopper Rail Hydraulic Train Buffer stopper | Seller: 834406 | 导轨缓冲塞 铁轨挡块是一种安全设备,安装在铁轨末端,以防止车辆打滑。具有结构合理、制动力大等特点。支撑臂和制动导轨采用插入式接头方式连接。它起源于中国,易于操作,维修和重置方便 | |
Small Stacker Autonomous Forklift | Seller: Shanghai Seer Intelligent Technology Corporation | Small Stacker Autonomous Forklift Robot downloadDOWNLOAD Narrow Aisles Exclusive, Versatile a... | |
See the latest cases | Seller: Shanghai Seer Intelligent Technology Corporation | Build My Botarrow-icon Mobile Robot Controllers Mobile Robot Controllers Peripheral Component... | |
Small Stacker Autonomous Forklift | Seller: Shanghai Seer Intelligent Technology Corporation | Small Stacker Autonomous Forklift Narrow Aisles Exclusive, Versatile and Universal Built-in... | |
Mobile Robot Controllers | Seller: Shanghai Seer Intelligent Technology Corporation | SRC-2000-F(S) This product is dedicated to the general laser SLAM navigation controller in the... |