25-OH Vitamin D

Vitamin D is converted to 25 hydroxy d vitamin(25ohd vitamin d) by hydroxylation in the liver and the active 1, 25-dihydroxy vitamin D in the kidneys.

25 hydroxyvitamin D is the main form of vitamin D in the body.

The level of serum 25 oh d vitamin dcan reflect the level of vitamin D storage and correlate with the clinical symptoms of vitamin D deficiency

Similar to other steroid hormones, 1, 25 oh vitamin d3regulates tissue cell differentiation and related gene expression, mainly regulating blood calcium level and affecting cell differentiation.

25-OH-VD has a high content in blood and a long half-life.

It is stable and is not directly affected by diet and lifestyle. Therefore, clinical measurement of 25 oh vitamin dcan reflect the proper level of vitamin D in vivo.

The performance date of 25-OH-VD Rapid Quantitative Test Kit (FIA)

Manufacturer	SEKBIO
Parameter	25-OH-VD Rapid Quantitative Test Kit (FIA)
Linearity	5~100ng/mL
Detection limit	5ng/mL
Benchmark product	Roche

Method Comparison

Sample	Roche	Sekbio	Sample	Roche	Sekbio
1			31
2			32
3			33
4			34
5			35
6			36
7			37
8			38
9			39
10			40
11			41
12			42
13			43
14			44
15			45
16			46
17			47
18			48
19			49
20			50
21			51
22			52
23			53
24			54
25			55
26			56
27			57
28			58
29			59
30			60

Linear

Cal	Concentration	Result	AVERAGE
S1
S2
S3
S4
S5
S6

Accuracy

Cal	concentration	Result	Bias
A1
A2
			-7.3%
			-6.6%
A3			-5.9%

Precision

Cal	Concentration	Cal	Concentration	Cal	Concentration
R1		R2		R3
AVE		AVE		AVE
SD		SD		SD
CV		CV		CV

Vitamin D Products

Antibody	Application
Sheep Anti-human 25-OH Vitamin D mAb	For immunodiagnostic: ELISA, LFA, CLIA
Mouse Anti-human 25-OH Vitamin D mAb
Humanized Anti-human 25-OH Vitamin D mAb

VD product Introduction

Optimal Vitamin D status is determined by measuring the metabolite 25-OH vitamin D in the bloodstream. This form of vitamin D serves as the primary storage compound in the human body, significantly outweighing the biologically inactive circulating 1,25 (OH)2 vitamin D by approximately 1000-fold.

The circulating half-life of 25-OH vitamin D is approximately 2-3 weeks. It's worth noting that over 95% of measurable 25 0h vitamin din the serum consists of 25-OH vitamin D3, while 25ohd2 becomes detectable only in individuals who supplement with vitamin D2.

Vitamin D-binding protein

The human GCis approximately 58 kDa in size and consists of 458 amino acids. Its genetic code is comprised of 1690 nucleotides located on chromosome 4 (4q11–q13). A notable feature of GC is its primary structure, which includes 28 cysteine residues forming multiple disulfide bonds. The protein is divided into three domains: domain 1 comprising 10 alpha helices, domain 2 consisting of 9 helices, and domain 3 with 4 helices.

Interestingly, the metabolism of these domains appears to differ, particularly in their interaction with vitamin D. Research indicates that the vitamin D binding protein, a component of GC, may exhibit a higher affinity for 25(OH)D3 (the metabolite of vitamin D3) compared to 25(OH)D2 (the metabolite of vitamin D2). This finding was observed in a specific study.

Six point calibration test results
	RLU		AVER	CV	S/N
	1988864	1989951	1989408
	1701638	1717603	1709621
	1199394	1201402	1200398
	680993	677204	679099
	312820	318660	315740
	147914	146062	146988

Precision

	Diluent Ratio	RLU			AVE	CV	CalculatedConc.
		1701378	1678029	1682826	1687411
		705844	700950	722856	709883
		376481	382996	386917	382131
		249100	248073	255330	250834
		189357	191251	188225	189611
600		147871	146779	150422	148357

Repeatability

Sample	RLU	C1	RLU	C2
Repeat 20 times	1221777		316434
	1218254		316700
	1199261		325984
	1180962		324222
	1193284		313327
	1210626		315721
	1186392		310303
	1217894		328118
	1192191		319156
	1214137		320196
AVE
SD
CV≤8%

25-OH Vitamin D Intro

25-hydroxyvitamin D is the main form of vitamin D in the body. Vitamin D is a steroid derivative, is a fat-soluble vitamin, and is a cyclopentane poly hydro phenanthrene compound. Vitamin D is mainly synthesized by human skin after being irradiated by ultraviolet rays, and a small part is ingested from food or supplements. Vitamin D affects calcium and phosphorus metabolism and has a wide range of physiological effects. It is an indispensable substance for maintaining human health, cell growth, and development and is closely related to various diseases. There are two forms of vitamin D in the human body, vitamin D3 (cholecalciferol) and vitamin D2 (ergocalciferol), which are converted to 25-hydroxyvitamin D (25-OH-VD) by hydroxylation in the liver, which is then converted to active 1,25-dihydroxy vitamin D in the kidneys. The serum 25-OH-VD can reflect human vitamin D storage level, which is related to the clinical symptoms of vitamin D deficiency.

Vitamin D Antibody

Vitamin D is an important hormone that regulates calcium and phosphorus metabolism in the body. The skin mainly synthesizes it under sunlight conditions, and a small amount can obtain through diet. The active form of vitamin D is especially 1,25 dihydroxy vitamin d3 antibody(1,25(OH) 2 D 3 ), which exerts physiological effects by binding to the vitamin D receptor (VDR). 25-hydroxyvitamin D (25(OH)D) is the body's primary storage form of vitamin D. 1,25(OH)D3 induces the differentiation of monocytes into macrophages and reduces the release of inflammatory cells and chemokines.

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