Biopharmaceutical Equipment and Devices
Hanbon is a manufacturer of liquid ssmb chromatographyseries products with a large production scale and a complete range of products in China.
The main products include: Biopharm purification equipment, Oligo synthesizer, industrial preparation simulated moving bed smb chromatographysystems, supercritical fluid chromatography systems, continuous chromatography systems, laboratory chromatography instruments, etc., and can provide users with integrated solutions and services centered on chromatographic products.
Synthesis Principle Of Oligonucleotide
Most oligonucleotides are currently synthesized by solid-phase synthesis, using automated equipment, in which phosphoramidite monomers are required. For example, the phosphoramidite monomer for DNA synthesis mainly consists of bases, deoxyribose, 2-cyanoethyl 5'-DMT and 3'-terminus, and diisopropylamine group, while the phosphoramidite monomer for RNA requires protection of 2'-OH to prevent cross-reaction between 2'-OH and 5'-OH in solid-phase synthesis, and the protecting group is usually TBDMS. Solid-phase synthesis is frequently used nowadays, the traditional solid-phase carriers were controlled microporous glass beads (CPG), but the loading capacity was limited, only 70-80 μmol/g. Nowadays, more high loading capacity solid-phase carriers are used, and the loading capacity is generally up to 250umol/g~350umol/g.
Purification Method Of Oligonucleotide
Oligonucleotides are short-stranded nucleic acids consisting of less than 20 nucleotide units, and purification methods can be selected according to different oligonucleotide lengths, sequences, structures, and purity requirements. The following are some common oligonucleotide purification methods:
Above are some common methods for oligonucleotide purification, Hanbon can offer relevant equipment, which can be used during the RP-HPLC
Choose Hanbon for Your Biopharmaceutical Process
In the synthesis stage, we can provide different sizes of synthesizers to meet your needs in different stages of optimization and next step scale-up, from laboratory level to industrial production level;
In the purification stage, whether you choose reversed-phase chromatography or ion exchange methods, we have the corresponding equipment for you to choose from;
In the ultrafiltration stage, we can also provide different sizes of equipment to meet your needs for salt exchange concentration.
In a word, with Hanbon, you can find the system that matches your need for your production process.
For more informationabout simulated moving bed adsorptionand simulated moving bed chromatography principle, please feel free to contact us!
Send product request
Other supplier products
 |
Continuous Ion Exchange Chromatography | Continuous dissociation technology is developed by combining the traditional fixed resin bed technology with the continuous counter-current system.... |
 |
Buffer Dilution/ Conditioning System | Bio-Con® auto dilution systemis to mix various concentrated buffer solutions, including acid, alkali, salt, etc., with injection water online t... |
 |
DAC® Dynamic Axial Compression Column | Dynamic axial compression columnare the most mature areas of preparative chromatography using the technology of packing column, dynamic axial compr... |
 |
Continuous Chromatographic System | Continuous chromatography systems By implementing this approach, the utilization rate of the ion exchange column can be enhanced, resulting in inc... |
 |
Bio-TFF Tangential Flow Filtration System | Bio-TFF tangential flow filtration tff systemis an easy-to-use automatic ultrafiltration UF/DF system suitable for biopharmaceutical process develo... |
Same products
|
|
Biopharmaceutical Equipment and Devices | Seller: Jiangsu Hanbon Science&Technology Co., LTD | Hanbon is a manufacturer of liquid ssmb chromatographyseries products with a large production sca... |
 |
SFC-Dynamic Axial Compression Column | Seller: Jiangsu Hanbon Science&Technology Co., LTD | SFC-Dynamic Axial Compression Column (DAC) has the most mature loading technology in the field of... |
 |
Preparative Liquid Chromatography System | Seller: Jiangsu Hanbon Science&Technology Co., LTD | Hanbon preparative liquid chromatography system has the characteristics of high efficiency, high ... |
 |
Preparative HPLC | Seller: Jiangsu Hanbon Science&Technology Co., LTD | Hanbon offers Hanbon preparative HPLCand MPLC from laboratory to industrial level, which are esse... |
 |
Oligo Synthesizer | Seller: Jiangsu Hanbon Science&Technology Co., LTD | Bio-Oligo synthesizer machineis an efficient, rapid and reliable device for oligonucleotide synth... |
