Countstar® Rigel S2 Fluorescence Cell Analyzer

Precise cell analysis for:

• PBMCs, whole blood, Car-T
• Stem Cell Analysis
• Splenocytes
• Monocytes
• And Other Primary Cells

Product Overview
TheCountstarS2 System combines the two fluorescence channels plus a bright field digital microscope, an image cytometer, and a cell counter in a single bench-top instrument. This application-driven, compact, automated cell imaging system provides an all-in-one solution for cell counting, cell viability and Cell transfection efficiency through the use of preconfigured biological applications (BioApps). They are specifically optimized for analysis of primary cells from peripheral blood, including PBMC, CAR-T, NK cell and MSC, which commonly used in cell therapy.

Excitation light: 480nm, 525nm
Emission filter：535nm, 600LP

Key Benefits

• Automatic detecting 5 samples for one time
• GMP and 21 CFR Part 11 ready
• Multi channel, multi functions
• Simple, effortless operation
• Maintenance and service free
• All in one design- Touch screen user interface
• User friendly and flexible software

Smart
The BioApps of the Countstar Rigel S2 System simplify routine cell lab tasks while providing high quality scientific data.
Countstar S2 comes with three BioApps to simplify and automate the routine cell count, viability and cytotoxicity tasks.
Trypan blue BioApp: Obtain cell count, viability and concentration estimations based on trypan blue staining using a disposable consumable.
AO/PI Viability BioApp: Run two fluorescence color assays in disposable consumable to determine the percentages of live, dead cells and concentration in the presents of debris and unwanted nonnucleated cell types including red blood cells.
GFP transfection: The green fluorescent protein (GFP) exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. This protocol can analyze counting and percentage of GFP.


Three step process

Reproducible and Accurate

Touch Screen with Flexible Experiment Assay Management System

GMP ready

Applications
Monitoring the Quality of CAR-T Production during the whole Process

The immuno therapy, such as CAR-T involves the collection of peripheral blood mononuclear cells (PBMC) from the patient, then enrichment of the desired T cell subset followed by genetic modification to create engineered CAR-T cells. The CAR-T cells are expanded in large-scale and then infused back into the patient. With the Countstar S2 system it is possible to continuously monitor the viability and concentration every step of the entire process of manufacturing CAR-T cells under GMP.

Dual-Fluorescence Viability

GFP Transfection Efficiency
In cell and molecular biology, the GFP gene is frequently used as a reporter of expression. Currently, scientists are commonly using the fluorescent microscopes or flow cytometers to analyze the transfection efficiency of mammalian cells. But Flow cytometer requires a high-qualified and experienced operator. While Countstar FL enables users to get the result of a transfection efficiency assay quickly and accurately.

Trypan blue viability and concentration
Trypan blue viability and concentration assay also can be available on Countstar S2. Shown here, are the enlarged representative images of cell line. Since all of the cells were stained with trypan blue, we show the stained trypan blue image as well as the Countstar S2 counted image.