BlueGene Biotech Canine BCL2 Associated X Protein ELISA kit
ShanghaiBlueGene Biotech
Canine BCL2 Associated X Protein ELISA kit
E08B0032 Canine BCL2 Associated X Protein ELISA kit
The Canine BCL2 Associated X Protein ELISA kit is suitable for the detection of samples from Canine species. BCL2 Associated X Protein can also be called BAX, BCL2L4, Bax Zeta, Apoptosis regulator.
Specifications of Canine BCL2 Associated X Protein ELISA kit
|
Product Information |
|
|
E08B0032 |
|
|
Product Name |
Canine BCL2 Associated X Protein ELISA kit |
|
Species |
Canine |
|
Product Size |
48 Tests / 96 Tests |
|
Concentration |
5-100 ng/mL |
|
Sensitivity |
|
|
Principal |
Competitive ELISA |
|
Sample Volume |
100 ul |
|
Sample Type |
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
|
Assay Time |
90 minutes |
|
Platform |
Microplate Reader |
|
Conjugate |
HRP |
|
Detection Method |
Colorimetric |
|
Storage |
2-8°C |
|
Kit Components |
||
|
MATERIALS |
SPECIFICATION |
QUANTITY |
|
MICROTITER PLATE |
96 wells |
stripwell |
|
ENZYME CONJUGATE |
1 vial |
|
|
STANDARD A (0.5mL) |
0 ng/mL |
1 vial |
|
STANDARD B (0.5mL) |
5 ng/mL |
1 vial |
|
STANDARD C (0.5mL) |
10 ng/mL |
1 vial |
|
STANDARD D (0.5mL) |
25 ng/mL |
1 vial |
|
STANDARD E (0.5mL) |
50 ng/mL |
1 vial |
|
STANDARD F (0.5mL) |
100 ng/mL |
1 vial |
|
SUBSTRATE A |
6 mL |
1 vial |
|
SUBSTRATE B |
6 mL |
1 vial |
|
STOP SOLUTION |
6 mL |
1 vial |
|
WASH SOLUTION (100 x) |
10 mL |
1 vial |
|
BALANCE SOLUTION |
3 mL |
1 vial |
Principle of the Assay
BAX ELISA kitapplies the competitive enzyme immunoassay technique utilizing an anti-BAX antibody and an BAX-HRP conjugate. The assay sample and buffer are incubated together with BAX-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BAX concentration since BAX from samples and BAX-HRP conjugate compete for the anti-BAX antibody binding site. Since the number of sites is limited, as more sites are occupied by BAX from the sample, fewer sites are left to bind BAX-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BAX concentration in each sample is interpolated from this standard curve.
Quality Control on Canine BCL2 Associated X Protein ELISA BAXkit
|
Coefficient of Variance |
Intra Variation% ＜10% |
|
|
Inter Variation% ＜12% |
||
|
Recovery |
90-104% |
|
|
Linearity |
Diluent Ratio |
Range % |
|
1:02 |
89-105 |
|
|
1:04 |
87-106 |
|
|
1:08 |
84-109 |
|
|
Specificity/Cross-reactivity |
No significant cross-reactivity or interference between BAX and analogues was observed. |
|
Citations of Canine BCL2 Associated X Protein ELISA kit
E08B0032 has been referenced in the below publications:
Articular Cartilage Gene Expression after Coxofemoral Joint Luxation in the Dog.
We can also offer sorts of cho host cell protein elisa kitfor sale, if you have needs, please contact us.
Send product request
Other supplier products
 |
BlueGene Biotech Porcine Diamine Oxidase ELISA kit | E07D0032 Porcine Diamine Oxidase ELISA kit The Porcine Diamine Oxidase ELISA kit is suitable for detecting samples from porcine species. Diamine... |
 |
Human Asymmetric Dimethylaoyoinine ELISA kit | E01A0035 Human Asymmetric Dimethylaoyoinine ELISA kit Human Asymmetric Dimethylaoyoinine ADMA ELISAkit is suitable for the detection of samples ... |
 |
BlueGene Biotech Guinea Pig Eosinophil Cationic Protein ELISA kit | E05E0125 Guinea Pig Eosinophil Cationic Protein ELISAkit Guinea Pig Eosinophil cationic protein ELISA kit is suitable for the detection of sampl... |
 |
ELISA Kits | The elisa kit companyShanghai BlueGene Biotechhas focused on the research and development of ELISA kits, especially for more than a decade. Our ... |
 |
Human Adrenomedullin ELISA kit | E01A0047 Human Adrenomedullin ELISA kit Human Adrenomedullin ELISAkit is suitable for the detection of samples from human species. Adrenomedulli... |
Same products
 |
BlueGene Biotech Sheep Cluster of Differentiation 4 ELISA kit | Seller: Bluegene Biotech | E14C0004 Sheep Cluster of Differentiation 4 ELISA kit Sheep Cluster of Differentiation 4 ELISA... |
 |
BlueGene Biotech Sheep Anti Interleukin 10 ELISA kit | Seller: Bluegene Biotech | Specifications of Sheep Anti Interleukin 10 ELISAkit Product Information E1... |
 |
BlueGene Biotech Chicken Total Adiponectin ELISA kit | Seller: Bluegene Biotech | E12A0125 Chicken Total Adiponectin ELISA kit Chicken Total Adiponectin ELISA kit (a knid of ch... |
 |
BlueGene Biotech Bovine Decorin ELISA kit | Seller: Bluegene Biotech | E11D0145 Bovine Decorin ELISA kit Bovine Decorin ELISAkit is suitable for the detection of sam... |
 |
BlueGene Biotech Bovine C Type Natriuretic Peptide ELISA kit | Seller: Bluegene Biotech | E11C0339 Bovine C Type Natriuretic PeptideELISA kit Bovine C Type Natriuretic Peptide ELISAkit... |
