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BlueGene Biotech Sheep Anti Interleukin 10 ELISA kit

Specifications of Sheep Anti Interleukin 10 ELISAkit

Product Information

E14A0081

Product Name

Sheep Anti Interleukin 10 ELISA kit

Species

Sheep

Product Size

48 Tests / 96 Tests

Concentration

25-500 ng/ml

Sensitivity

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C

Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

25 ng/mL

1 vial

STANDARD C (0.5mL)

50 ng/mL

1 vial

STANDARD D (0.5mL)

100 ng/mL

1 vial

STANDARD E (0.5mL)

250 ng/mL

1 vial

STANDARD F (0.5mL)

500 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial

Principle of the Assay

Anti-il 10 elisaapplies the competitive enzyme immunoassay technique utilizing IL 10 antigen and an Anti-IL 10-HRP conjugate. The assay sample and buffer are incubated together with Anti-IL 10-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Anti-IL 10 concentration since Anti-IL 10 from samples and Anti-IL 10-HRP conjugate compete for the IL 10 antigen binding site. Since the number of sites is limited, as more sites are occupied by Anti-IL 10 from the sample, fewer sites are left to bind Anti-IL 10-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Anti-IL 10 concentration in each sample is interpolated from this standard curve.

Quality Control on Sheep Anti Interleukin 10 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% 12%

Recovery

87-118.37%

Linearity

Diluent Ratio

Range %

1:2

98-117

1:4

93-106

1:8

91-105

Specificity/Cross-reactivity

No significant cross-reactivity or interference between Anti IL 10 and analogues was observed.

Bluegene Biotech has been committed to R&D of ELISA kits, making our ELISA kits more convenient for researchers to use on the premise of guaranteeing the quality, thus assisting scientific research for our customers. So far, we have received 1200 published papers from researchers with a total of about 500 kinds of ELISA Kits that have been cited. Bluegene ELISA kithas the advantages of high specificity, high sensitivity, and high stability.

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