BlueGene Biotech Bovine Catalase ELISA kit
E11C0086 Bovine Catalase ELISA kit
Bovine Catalase ELISA kitis suitable for the detection of samples from Bovine species. Catalase can also be called Cas1, CAT, Cs1.
Specifications of Bovine Catalase ELISAkit
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Product Information |
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E11C0086 |
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Product Name |
Bovine Catalase ELISA kit |
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Species |
Bovine |
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Product Size |
48 Tests / 96 Tests |
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Concentration |
10-250 ng/mL |
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Sensitivity |
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Principal |
Competitive ELISA |
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Sample Volume |
100 ul |
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Sample Type |
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
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Assay Time |
90 minutes |
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Platform |
Microplate Reader |
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Conjugate |
HRP |
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Detection Method |
Colorimetric |
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Storage |
2-8°C |
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Kit Components |
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MATERIALS |
SPECIFICATION |
QUANTITY |
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MICROTITER PLATE |
96 wells |
stripwell |
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ENZYME CONJUGATE |
1 vial |
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STANDARD A (0.5mL) |
0 ng/mL |
1 vial |
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STANDARD B (0.5mL) |
10 ng/mL |
1 vial |
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STANDARD C (0.5mL) |
25 ng/mL |
1 vial |
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STANDARD D (0.5mL) |
50 ng/mL |
1 vial |
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STANDARD E (0.5mL) |
100 ng/mL |
1 vial |
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STANDARD F (0.5mL) |
250 ng/mL |
1 vial |
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SUBSTRATE A |
6 mL |
1 vial |
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SUBSTRATE B |
6 mL |
1 vial |
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STOP SOLUTION |
6 mL |
1 vial |
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WASH SOLUTION (100 x) |
10 mL |
1 vial |
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BALANCE SOLUTION |
3 mL |
1 vial |
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Principle of the Assay |
CAT ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-CAT antibody and an CAT-HRP conjugate. The assay sample and buffer are incubated together with CAT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CAT concentration since CAT from samples and CAT-HRP conjugate compete for the anti-CAT antibody binding site. Since the number of sites is limited, as more sites are occupied by CAT from the sample, fewer sites are left to bind CAT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CAT concentration in each sample is interpolated from this standard curve.
Quality Control on Bovine Catalase ELISA kit
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Coefficient of Variance |
Intra Variation% ＜10% |
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Inter Variation% ＜12% |
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Recovery |
91-105% |
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Linearity |
Diluent Ratio |
Range % |
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1:2 |
87-108 |
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1:4 |
89-109 |
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1:8 |
85-111 |
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Specificity/Cross-reactivity |
No significant cross-reactivity or interference between CAT and analogues was observed. |
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As one of mouse igg elisa kitsmanufacturers, we are committed to providing the kits with simple and easy operations but accurate and reliable quality to the researchers, and we have won a good reputation from customers all around the world. Many published papers have cited the usage of BlueGene BiotechELISA kits.
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