Keratinase
Protease catalyzes peptide bond and hydrolyzes protein into peptone, keratinase enzyme, polypeptide and amino acid. As a kind of alkaline serine protease, keratinase not only has the function of normal alkaline protease, but also can break down disulfide bond and disintegrate keratin.
Definition and Dosage of Activity Keratinase
One unit (U) of protease activity is defined as the amount of enzyme which liberates 1 μg tyrosine per minute from substrate casein at 40℃ and pH 10.5 under the conditions of the test.
Recommended dosage is 50-100g/t with 50000 U/g in complete feed.
Functions of KeratinaseProducts
Application of Keratinase
- The matrix value of keratinase (100g/t)
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Crude protein, % |
5400 |
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Lysine, % |
150 |
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Methionine, % |
180 |
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Cystine, % |
150 |
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Threonine, % |
210 |
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Tryptophane, % |
90 |
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Isoleucine , % |
150 |
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Arginine, % |
285 |
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Valine, % |
255 |
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Alkaline Protease |
Alkaline protease works on most of the peptide bonds therefore can hydrolyze diversified proteins and save the protein resource. |
Experimental animal: 840 one-day-old AA broilers, 6×35 broilers/treatment.
Experimental design:
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Item |
Diet |
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PC |
Basal diet |
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NC |
Basal diet-0.5% crude protein |
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Group I |
NC+125g/t competitor |
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Group II |
NC+100g/t Keratinase(30000U/g) |
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Experimental cycle: 42 days |
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Results: |
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Table2 Effect of each treatment on the growth performance of AA broilers (g, %) |
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Items |
PC |
NC |
Group I |
Group II |
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Initial weight,g |
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1-21days |
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21stAG,g |
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ADFI,g |
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ADG,g |
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F/G |
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Death rate,% |
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22-42days |
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22ndAG,g |
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42ndAG,g |
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ADFI,g |
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ADG,g |
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F/G |
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Death rate,% |
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1-42days |
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ADFI,g |
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ADG,g |
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F/G |
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Death rate,% |
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Note: In the same row, values with different small letter superscripts mean significant difference(P<0.05).
Conclusion of Keratinase
On the basis of these results, it is concluded that the supplement of keratinase into a low CP diet was as effective as normal level in broiler. As shown in diagram 1, keratinaseimprove the digestibility of dry matter, crude protein, crude fat, general energy and apparent metabolic energy. And in table 4, karatinase has a positive effect on the amino acids digestibility. In this experiment, it is appeared that karatinase improve the growth performance of broiler by increase average diary gain and feed conversion rate.
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