Keratinase
Protease catalyzes peptide bond and hydrolyzes protein into peptone, keratinase enzyme, polypeptide and amino acid. As a kind of alkaline serine protease, keratinase not only has the function of normal alkaline protease, but also can break down disulfide bond and disintegrate keratin.
Definition and Dosage of Activity Keratinase
One unit (U) of protease activity is defined as the amount of enzyme which liberates 1 μg tyrosine per minute from substrate casein at 40℃ and pH 10.5 under the conditions of the test.
Recommended dosage is 50-100g/t with 50000 U/g in complete feed.
Functions of KeratinaseProducts
Application of Keratinase
- The matrix value of keratinase (100g/t)
|
Crude protein, % |
5400 |
|
|
Lysine, % |
150 |
|
|
Methionine, % |
180 |
|
|
Cystine, % |
150 |
|
|
Threonine, % |
210 |
|
|
Tryptophane, % |
90 |
|
|
Isoleucine , % |
150 |
|
|
Arginine, % |
285 |
|
|
Valine, % |
255 |
|
|
Alkaline Protease |
Alkaline protease works on most of the peptide bonds therefore can hydrolyze diversified proteins and save the protein resource. |
Experimental animal: 840 one-day-old AA broilers, 6×35 broilers/treatment.
Experimental design:
|
Item |
Diet |
||||
|
PC |
Basal diet |
||||
|
NC |
Basal diet-0.5% crude protein |
||||
|
Group I |
NC+125g/t competitor |
||||
|
Group II |
NC+100g/t Keratinase(30000U/g) |
||||
|
Experimental cycle: 42 days |
|||||
|
Results: |
|||||
|
Table2 Effect of each treatment on the growth performance of AA broilers (g, %) |
|||||
|
Items |
PC |
NC |
Group I |
Group II |
|
|
Initial weight,g |
|||||
|
1-21days |
|||||
|
21stAG,g |
|||||
|
ADFI,g |
|||||
|
ADG,g |
|||||
|
F/G |
|||||
|
Death rate,% |
|||||
|
22-42days |
|||||
|
22ndAG,g |
|||||
|
42ndAG,g |
|||||
|
ADFI,g |
|||||
|
ADG,g |
|||||
|
F/G |
|||||
|
Death rate,% |
|||||
|
1-42days |
|||||
|
ADFI,g |
|||||
|
ADG,g |
|||||
|
F/G |
|||||
|
Death rate,% |
|||||
Note: In the same row, values with different small letter superscripts mean significant difference(P<0.05).
Conclusion of Keratinase
On the basis of these results, it is concluded that the supplement of keratinase into a low CP diet was as effective as normal level in broiler. As shown in diagram 1, keratinaseimprove the digestibility of dry matter, crude protein, crude fat, general energy and apparent metabolic energy. And in table 4, karatinase has a positive effect on the amino acids digestibility. In this experiment, it is appeared that karatinase improve the growth performance of broiler by increase average diary gain and feed conversion rate.
Bestzyme Bio-Engineering Co., Ltd. is a professional enzyme supplier, we provide china enzyme, Alpha amylaseand etc. Want to know more, please contact us.
Send product request
Other supplier products
 |
Acid Protease | Protease catalyzes peptide bond and hydrolyzes protein into peptone, hydrazone, polypeptide and amino acid. Activity center of acid protease includ... |
 |
Beta Mannanse | βmannanaseis a feed hemicellulase fermented by the improved strains using a liquid fermentation technique, and produced by an advanced post-tr... |
 |
Feed Enzyme | Feed enzymeis a widely recognized feed additive, which includes Phytase, Non Starch Polysaccharides enzymes, functional enzyme. The functions of en... |
 |
Xylanase | Xylan is contained commonly in a variety of feed ingredients, like wheat, rye, corn, cereal by-products, soybean meal, etc. Whereas, xylanase enzym... |
 |
Alpha Galactosidase | Bestzyme Bio-Engineering Co., Ltd. is a enzyme supplier, focusing on research, development, manufacturing of enzyme products and application know-h... |
Same products
 |
Face Makeup Supplier | Seller: Guangzhou Doriff Cosmetics Co., Ltd. | Face Makeup Supplier Face Makeup Doriff is one of the largest FDA, GMPC, ISO and MSDS cer... |
 |
Pectinase | Seller: Jinan Bestzyme Bio-Engineering Co., Ltd | Pectinase for Sale This product is a mixture of pectinose and pectinase which are obtained by As... |
 |
Neutral Protease | Seller: Jinan Bestzyme Bio-Engineering Co., Ltd | Neutral Protease Neutral protease catalyzes peptide bond and hydrolyzes protein into peptone, hy... |
 |
Applications | Seller: Jinan Bestzyme Bio-Engineering Co., Ltd | Beta Glucanase Enzyme Uses Bestzyme enzymes are widely used in feed and industrial fields. For i... |
 |
Beta Mannanase | Seller: Jinan Bestzyme Bio-Engineering Co., Ltd | β mannanase is a feed hemicellulase fermented by the improved strains using a liquid ferment... |
