.

Keratinase

Protease catalyzes peptide bond and hydrolyzes protein into peptone, keratinase enzyme, polypeptide and amino acid. As a kind of alkaline serine protease, keratinase not only has the function of normal alkaline protease, but also can break down disulfide bond and disintegrate keratin.

Definition and Dosage of Activity Keratinase

One unit (U) of protease activity is defined as the amount of enzyme which liberates 1 μg tyrosine per minute from substrate casein at 40℃ and pH 10.5 under the conditions of the test.

Recommended dosage is 50-100g/t with 50000 U/g in complete feed.

Functions of KeratinaseProducts

Application of Keratinase

  1. The matrix value of keratinase (100g/t)

Crude protein, %

5400

Lysine, %

150

Methionine, %

180

Cystine, %

150

Threonine, %

210

Tryptophane, %

90

Isoleucine , %

150

Arginine, %

285

Valine, %

255

Alkaline Protease

Alkaline protease works on most of the peptide bonds therefore can hydrolyze diversified proteins and save the protein resource.

Experimental animal: 840 one-day-old AA broilers, 6×35 broilers/treatment.

Experimental design:

Item

Diet

PC

Basal diet

NC

Basal diet-0.5% crude protein

Group I

NC+125g/t competitor

Group II

NC+100g/t Keratinase(30000U/g)

Experimental cycle: 42 days

Results:

Table2 Effect of each treatment on the growth performance of AA broilers (g, %)

Items

PC

NC

Group I

Group II

Initial weight,g

1-21days

21stAG,g

ADFI,g

ADG,g

F/G

Death rate,%

22-42days

22ndAG,g

42ndAG,g

ADFI,g

ADG,g

F/G

Death rate,%

1-42days

ADFI,g

ADG,g

F/G

Death rate,%

Note: In the same row, values with different small letter superscripts mean significant difference(P<0.05).

Conclusion of Keratinase

On the basis of these results, it is concluded that the supplement of keratinase into a low CP diet was as effective as normal level in broiler. As shown in diagram 1, keratinaseimprove the digestibility of dry matter, crude protein, crude fat, general energy and apparent metabolic energy. And in table 4, karatinase has a positive effect on the amino acids digestibility. In this experiment, it is appeared that karatinase improve the growth performance of broiler by increase average diary gain and feed conversion rate.

Bestzyme Bio-Engineering Co., Ltd. is a professional enzyme supplier, we provide china enzyme, Alpha amylaseand etc. Want to know more, please contact us.



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