Keratinase

Protease catalyzes peptide bond and hydrolyzes protein into peptone, keratinase enzyme, polypeptide and amino acid. As a kind of alkaline serine protease, keratinase not only has the function of normal alkaline protease, but also can break down disulfide bond and disintegrate keratin.

Definition and Dosage of Activity Keratinase

One unit (U) of protease activity is defined as the amount of enzyme which liberates 1 μg tyrosine per minute from substrate casein at 40℃ and pH 10.5 under the conditions of the test.

Recommended dosage is 50-100g/t with 50000 U/g in complete feed.

Functions of KeratinaseProducts

Application of Keratinase

1. The matrix value of keratinase (100g/t)

Crude protein, %	5400
Lysine, %	150
Methionine, %	180
Cystine, %	150
Threonine, %	210
Tryptophane, %	90
Isoleucine , %	150
Arginine, %	285
Valine, %	255
	Alkaline Protease	Alkaline protease works on most of the peptide bonds therefore can hydrolyze diversified proteins and save the protein resource.

Experimental animal: 840 one-day-old AA broilers, 6×35 broilers/treatment.

Experimental design:

Item	Diet
PC	Basal diet
NC	Basal diet-0.5% crude protein
Group I	NC+125g/t competitor
Group II	NC+100g/t Keratinase(30000U/g)
Experimental cycle: 42 days
Results:
Table2 Effect of each treatment on the growth performance of AA broilers (g, %)
Items	PC	NC	Group I		Group II
Initial weight,g
1-21days
21stAG,g
ADFI,g
ADG,g
F/G
Death rate,%
22-42days
22ndAG,g
42ndAG,g
ADFI,g
ADG,g
F/G
Death rate,%
1-42days
ADFI,g
ADG,g
F/G
Death rate,%

Note: In the same row, values with different small letter superscripts mean significant difference(P<0.05).

Conclusion of Keratinase

On the basis of these results, it is concluded that the supplement of keratinase into a low CP diet was as effective as normal level in broiler. As shown in diagram 1, keratinaseimprove the digestibility of dry matter, crude protein, crude fat, general energy and apparent metabolic energy. And in table 4, karatinase has a positive effect on the amino acids digestibility. In this experiment, it is appeared that karatinase improve the growth performance of broiler by increase average diary gain and feed conversion rate.

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