Rat Alpha Amylase ELISA kit

• 

E02A0010 Rat Alpha Amylase ELISA kit

Rat Alpha Amylase ELISAkit is suitable for the detection of samples from Rat species. Alpha Amylase can also be called glycogenase, α amylase, endoamylase, Taka-amylase A, 1,4-α-D-glucan glucanohydrolase, AMY.

Principle of the Assay

AMY ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-AMY antibody and an AMY-HRP conjugate. The assay sample and buffer are incubated together with AMY-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AMY concentration since AMY from samples and AMY-HRP conjugate compete for the anti-AMY antibody binding site. Since the number of sites is limited, as more sites are occupied by AMY from the sample, fewer sites are left to bind AMY-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AMY concentration in each sample is interpolated from this standard curve.

Citations of Rat Alpha Amylase ELISA kit

E02A0010 has been referenced in the below publications:

The protective effect and mechanism of the alanyl-glutamine dipeptide in the treatment of severe acute pancreatitis.

As one insulinelisakit manufacturer, we are committed to providing the kits with simple and easy operations but accurate and reliable quality to the researchers, and we have won a good reputation from customers all around the world. Many published papers have cited the usage of BlueGeneBiotech ELISA kits. In addition, we provide a 100% Risk-Free Performance Guarantee, professional technical service, and timely assistance for all customers. Our strict and sound quality control is a key part to sustainably supply our products for the international market for more than 12 years. More new products with good quality are coming soon...

Другие товары поставщика

	Hamster ELISA Kits	Hamster ELISA Kits A hamster is also known as suslik, which is an animal domesticated by wild animals and entered the laboratory. There are 8 kind...
	Rat Alpha Amylase ELISA kit	E02A0010 Rat Alpha Amylase ELISA kit Rat Alpha Amylase ELISAkit is suitable for the detection of samples from Rat species. Alpha Amylase can als...
	BlueGene Biotech Canine Brain Derived Neurotrophic Factor ELISA kit	E08B0029 Canine Brain Derived Neurotrophic Factor ELISA kit Canine Brain Derived Neurotrophic Factor BDNFELISA kitis suitable for the detection ...
	Human Adrenomedullin ELISA kit	E01A0047 Human Adrenomedullin ELISA kit Human Adrenomedullin ELISAkit is suitable for the detection of samples from human species. Adrenomedulli...
	Fish ELISA Kits	Fish ELISA Kits Fish are often used in environmental monitoring, embryo research, tumor research and others. Among them, zebrafish is the most com...

Похожие товары

	Chlorine dioxide powder	Продавец: Shanghai Shicun Industrial Co., Ltd.	Chlorine dioxide powder Chemical formula: ClO2Molecular weight: 67.452CAS:10049-04-4EINECS:233-1...
	Sodium polyacrylate	Продавец: Shanghai Shicun Industrial Co., Ltd.	Sodium polyacrylate What is Sodium Polyacrylate? Sodium polyacrylate, chemical formula(C3H3NaO2...
	Thermal conductive silicone	Продавец: Shanghai Shicun Industrial Co., Ltd.	Thermal conductive silicone Thermal conductive silicone is a high-performance material designed ...
	Palm oil fatty acids	Продавец: Shanghai Shicun Industrial Co., Ltd.	Palm oil fatty acids As a leadingTrusted Palm Oil Fatty Acids Supplier, we offer premium-gradeFa...
	Peptide Technology Cooperation & Transfer	Продавец: PeptiOrigin Biotechnology Co., Ltd.	Peptide Technology Cooperation & Transfer Driving Innovation Through Strategic Partnerships ...