Rat Alpha Amylase ELISA kit

E02A0010 Rat Alpha Amylase ELISA kit

Rat Alpha Amylase ELISAkit is suitable for the detection of samples from Rat species. Alpha Amylase can also be called glycogenase, α amylase, endoamylase, Taka-amylase A, 1,4-α-D-glucan glucanohydrolase, AMY.

Principle of the Assay

AMY ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-AMY antibody and an AMY-HRP conjugate. The assay sample and buffer are incubated together with AMY-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AMY concentration since AMY from samples and AMY-HRP conjugate compete for the anti-AMY antibody binding site. Since the number of sites is limited, as more sites are occupied by AMY from the sample, fewer sites are left to bind AMY-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AMY concentration in each sample is interpolated from this standard curve.

Citations of Rat Alpha Amylase ELISA kit

E02A0010 has been referenced in the below publications:

The protective effect and mechanism of the alanyl-glutamine dipeptide in the treatment of severe acute pancreatitis.

As one insulinelisakit manufacturer, we are committed to providing the kits with simple and easy operations but accurate and reliable quality to the researchers, and we have won a good reputation from customers all around the world. Many published papers have cited the usage of BlueGeneBiotech ELISA kits. In addition, we provide a 100% Risk-Free Performance Guarantee, professional technical service, and timely assistance for all customers. Our strict and sound quality control is a key part to sustainably supply our products for the international market for more than 12 years. More new products with good quality are coming soon...

Other supplier products

	BlueGene Biotech Porcine Total Adiponectin ELISA kit	BlueGene Biotech Porcine Total Adiponectin ELISAkit Adiponectin can also be called 30 kDa adipocyte complement related protein, 30 kDa adipocyte ...
	BlueGene Biotech Sheep Anti Interleukin 10 ELISA kit	Specifications of Sheep Anti Interleukin 10 ELISAkit Product Information E14A0081 Product Name Sheep Anti Inter...
	BlueGene Biotech Porcine Diamine Oxidase ELISA kit	E07D0032 Porcine Diamine Oxidase ELISA kit The Porcine Diamine Oxidase ELISA kit is suitable for detecting samples from porcine species. Diamine...
	Human Chemokine (C-C motif) Ligand 18 ELISA kit	Immunology NE01C0064 Human Chemokine (C-C motif) Ligand 18 ELISA Kit Human Chemokine (C-C motif) ligand ccl18 elisakit is suitable for the dete...
	BlueGene Biotech Guinea Pig Eosinophil Cationic Protein ELISA kit	E05E0125 Guinea Pig Eosinophil Cationic Protein ELISAkit Guinea Pig Eosinophil cationic protein ELISA kit is suitable for the detection of sampl...

供应产品

Same products

	Water Purification Equipment	卖方: Dongguan Lede New Material Technology Co., Ltd	Water Purification Equipment Pure water (deionized water)is required at three critical points in...
	Fully Automatic Vertical Lifting Electrophoretic Coating Line	卖方: Dongguan Lede New Material Technology Co., Ltd	Fully Automatic Vertical Lifting Electrophoretic Coating Line LENDEER'sfullyautomatic vertical l...
	Semi-Automatic Lifting Immersion Electrophoretic Coating Production Line	卖方: Dongguan Lede New Material Technology Co., Ltd	Semi-Automatic Lifting Immersion Electrophoretic Coating Production Line LENDEER'ssemi-automatic...
	Semi-automatic hoist electrophoretic coating line	卖方: Dongguan Lede New Material Technology Co., Ltd	Semi-automatic hoist electrophoretic coating line A defining engineering feature of this line is...
	Cathodic White ED Paint	卖方: Dongguan Lede New Material Technology Co., Ltd	Cathodic White ED Paint 140°C Low-Temperature Cathodic White ED Paint LENDEERcathodic white...