BlueGene Biotech Guinea Pig Eosinophil Cationic Protein ELISA kit

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E05E0125 Guinea Pig Eosinophil Cationic Protein ELISAkit

Guinea Pig Eosinophil cationic protein ELISA kit is suitable for the detection of samples from guinea Pig species. Eosinophil cationic protein can also be called ribonuclease 3, Rnase-A3, RNASE3, ECP, RNS3, RAF1, ribonuclease A family member 3.

Principle of the Assay

ECP ELISAkit applies the competitive enzyme immunoassay technique utilizing an anti-ECP antibody and an ECP-HRP conjugate. The assay sample and buffer are incubated together with ECP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ECP concentration since ECP from samples and ECP-HRP conjugate compete for the anti-ECP antibody binding site. Since the number of sites is limited, as more sites are occupied by ECP from the sample, fewer sites are left to bind ECP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ECP concentration in each sample is interpolated from this standard curve.

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