BlueGene Biotech
Bovine Carnitine Palmitoyltransferase I ELISA kit
Metabolism
E11C0055 Bovine Carnitine Palmitoyltransferase IELISA kit
Bovine Carnitine Palmitoyltransferase I ELISA kit is suitable for the detection of samples from Bovine species. Carnitine palmitoyltransferase I can also be called arnitine acyltransferase I, CPTI, CAT1, CoA, carnitine acyltransferase i(CCAT), or palmitoyl CoA transferase I, CPT 1, CPT-1.
Specifications of Bovine Carnitine Palmitoyltransferase I ELISA kit
Product Information |
|
E11C0055 |
|
Product Name |
Bovine Carnitine palmitoyltransferase I ELISA kit |
Species |
Bovine |
Product Size |
48 Tests / 96 Tests |
Concentration |
|
Sensitivity |
|
Principal |
Competitive ELISA |
Sample Volume |
100 ul |
Sample Type |
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time |
90 minutes |
Platform |
Microplate Reader |
Conjugate |
HRP |
Detection Method |
Colorimetric |
Storage |
2-8°C |
Kit Components |
||
MATERIALS |
SPECIFICATION |
QUANTITY |
MICROTITER PLATE |
96 wells |
stripwell |
ENZYME CONJUGATE |
1 vial |
|
STANDARD A (0.5mL) |
0 ng/mL |
1 vial |
STANDARD B (0.5mL) |
1 vial |
|
STANDARD C (0.5mL) |
5 ng/mL |
1 vial |
STANDARD D (0.5mL) |
10 ng/mL |
1 vial |
STANDARD E (0.5mL) |
25 ng/mL |
1 vial |
STANDARD F (0.5mL) |
50 ng/mL |
1 vial |
SUBSTRATE A |
6 mL |
1 vial |
SUBSTRATE B |
6 mL |
1 vial |
STOP SOLUTION |
6 mL |
1 vial |
WASH SOLUTION (100 x) |
10 mL |
1 vial |
BALANCE SOLUTION |
3 mL |
1 vial |
Principle of the Assay |
The CPT 1 ELISA kit uses an anti-CPT 1 antibody and a CPT 1-HRP conjugate to perform a competitive enzyme immunoassay. The CPT 1-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CPT 1 concentration since CPT 1 from samples and CPT 1-HRP conjugate compete for the anti-CPT 1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CPT 1 from the sample, fewer sites are left to bind CPT 1-HRP conjugate. A standard curve is drawn connecting the color's intensity (O.D.) and standard concentration. From this standard curve, the CPT 1 concentration in each sample is extrapolated.
As one of human igg elisa kitsmanufacturers, we are committed to providing the kits with simple and easy operations but accurate and reliable quality to the researchers, and we have won a good reputation from customers all around the world. Many published papers have cited the usage of Shanghai BlueGene BiotechELISA kits.
Отправить запрос, связаться с поставщиком
Другие товары поставщика
BlueGene Biotech Bovine Decorin ELISA kit | E11D0145 Bovine Decorin ELISA kit Bovine Decorin ELISAkit is suitable for the detection of samples from Bovine species. Decorin can also be call... | |
BlueGene Biotech Porcine Cortisol ELISA kit | E07C0008 Porcine Cortisol ELISA kit The Porcine cort elisakit is suitable for detecting samples from porcine species. Cortisol can also be calle... | |
BlueGene Biotech Mouse Angiotensin 2 ELISA kit | E03A0204 Mouse Ang2 ELISAkit Mouse Angiotensin 2 ELISA kitis suitable for the detection of samples from mouse species. Angiotensin 2 can also be... | |
Fish ELISA Kits | Fish ELISA Kits Fish are often used in environmental monitoring, embryo research, tumor research and others. Among them, zebrafish is the most com... | |
BlueGene Biotech Porcine Total Adiponectin ELISA kit | BlueGene Biotech Porcine Total Adiponectin ELISAkit Adiponectin can also be called 30 kDa adipocyte complement related protein, 30 kDa adipocyte ... |
Похожие товары
BlueGene Biotech Sheep Cluster of Differentiation 4 ELISA kit | Продавец: Bluegene Biotech | E14C0004 Sheep Cluster of Differentiation 4 ELISA kit Sheep Cluster of Differentiation 4 ELISA... | |
BlueGene Biotech Sheep Anti Interleukin 10 ELISA kit | Продавец: Bluegene Biotech | Specifications of Sheep Anti Interleukin 10 ELISAkit Product Information E1... | |
BlueGene Biotech Chicken Total Adiponectin ELISA kit | Продавец: Bluegene Biotech | E12A0125 Chicken Total Adiponectin ELISA kit Chicken Total Adiponectin ELISA kit (a knid of ch... | |
BlueGene Biotech Bovine Decorin ELISA kit | Продавец: Bluegene Biotech | E11D0145 Bovine Decorin ELISA kit Bovine Decorin ELISAkit is suitable for the detection of sam... | |
BlueGene Biotech Bovine C Type Natriuretic Peptide ELISA kit | Продавец: Bluegene Biotech | E11C0339 Bovine C Type Natriuretic PeptideELISA kit Bovine C Type Natriuretic Peptide ELISAkit... |