BlueGene Biotech Porcine Diamine Oxidase ELISA kit
E07D0032 Porcine Diamine Oxidase ELISA kit
The Porcine Diamine Oxidase ELISA kit is suitable for detecting samples from porcine species. Diamine Oxidase can also be called Amiloride Binding Protein 1, ABP1, AOC1, KAO, Histaminase, Kidney amine oxidase, Amiloride-sensitive amine oxidase, Amine oxidase copper domain-containing protein 1.
Principle of the Assay
DAO ELISAkit applies the competitive enzyme immunoassay technique utilizing an anti-DAO antibody and an DAO-HRP conjugate. The assay sample and buffer are incubated together with DAO-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DAO concentration since DAO from samples and DAO-HRP conjugate compete for the anti-DAO antibody binding site. Since the number of sites is limited, as more sites are occupied by DAO from the sample, fewer sites are left to bind DAO-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DAO concentration in each sample is interpolated from this standard curve
Citations of Porcine Diamine Oxidase ELISA abpkit
E07D0032 has been referenced in the below publications:
Low-protein diets supplemented with glutamic acid or aspartic acid ameliorates intestinal damage in weaned piglets challenged with hydrogen peroxide.
As a professional drug residue test kit factory, Bluegenehas high-quality meth residue test kitsfor sale. Welcome to contact us if needed.
Отправить запрос, связаться с поставщиком
Другие товары поставщика
Human Adrenomedullin ELISA kit | E01A0047 Human Adrenomedullin ELISA kit Human Adrenomedullin ELISAkit is suitable for the detection of samples from human species. Adrenomedulli... | |
BlueGene Biotech Bovine Catalase ELISA kit | E11C0086 Bovine Catalase ELISA kit Bovine Catalase ELISA kitis suitable for the detection of samples from Bovine species. Catalase can also be c... | |
BlueGene Biotech Canine β Cell Leukemia/Lymphoma 2 ELISA kit | E08B0033 Canine β Cell Leukemia/Lymphoma 2 ELISA kit Canine β Cell Leukemia/Lymphoma 2 ELISA kit is suitable for the detection of samp... | |
Human Amyloid β Protein 40 ELISA kit | E01A0049 Human Amyloid β Protein 40 ELISA kit Human Adenosine diphosphate ELISA kit is suitable for the detection of samples from human spe... | |
BlueGene Biotech | Bovine Carnitine Palmitoyltransferase I ELISA kit Metabolism E11C0055 Bovine Carnitine Palmitoyltransferase IELISA kit Bovine Carnitine Palmit... |
Похожие товары
Silicone defoamer, Fatty alcohol defoamer, Polyether defoamer, Mineral oil defoamer | Продавец: ISO certification Food Safety Production License of Anti-foaming agent Halal Certification | Our INVINO antifoams are versatile, catering to a wide range of industries including papermaking,... | |
Silicone defoamer, Fatty alcohol defoamer, Polyether defoamer, Mineral oil defoamer | Продавец: ISO certification Food Safety Production License of Anti-foaming agent Halal Certification | Our INVINO antifoams are versatile, catering to a wide range of industries including papermaking,... | |
порошок ГПМЦ | Продавец: Йида ГПМЦ | Гидроксипропилметилцеллюлоза (ГПМЦ) производится из очищенного хлопка после измельчения, затем ре... | |
UHMWPE Plant | Продавец: Hubei Sanli Fengxiang Technology Co., Ltd | Ultra high molecular weight polyethylene (hereinafter called as UHMWPE) is a linear structural po... | |
Formic acid production technology | Продавец: Hubei Sanli Fengxiang Technology Co., Ltd | There are totally four production processes forformic acid production, incl. Sodium Formate Proce... |