BlueGene Biotech Porcine Diamine Oxidase ELISA kit
E07D0032 Porcine Diamine Oxidase ELISA kit
The Porcine Diamine Oxidase ELISA kit is suitable for detecting samples from porcine species. Diamine Oxidase can also be called Amiloride Binding Protein 1, ABP1, AOC1, KAO, Histaminase, Kidney amine oxidase, Amiloride-sensitive amine oxidase, Amine oxidase copper domain-containing protein 1.
Principle of the Assay
DAO ELISAkit applies the competitive enzyme immunoassay technique utilizing an anti-DAO antibody and an DAO-HRP conjugate. The assay sample and buffer are incubated together with DAO-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DAO concentration since DAO from samples and DAO-HRP conjugate compete for the anti-DAO antibody binding site. Since the number of sites is limited, as more sites are occupied by DAO from the sample, fewer sites are left to bind DAO-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DAO concentration in each sample is interpolated from this standard curve
Citations of Porcine Diamine Oxidase ELISA abpkit
E07D0032 has been referenced in the below publications:
Low-protein diets supplemented with glutamic acid or aspartic acid ameliorates intestinal damage in weaned piglets challenged with hydrogen peroxide.
As a professional drug residue test kit factory, Bluegenehas high-quality meth residue test kitsfor sale. Welcome to contact us if needed.
在线联系供应商
Other supplier products
|
|
BlueGene Biotech Porcine Diamine Oxidase ELISA kit | E07D0032 Porcine Diamine Oxidase ELISA kit The Porcine Diamine Oxidase ELISA kit is suitable for detecting samples from porcine species. Diamine... |
 |
Human Angiopoietin 2 ELISA kit | NE01A0503 Human Angiopoietin 2 ELISA kit Human angiotensin ii elisa kitis suitable for the detection of samples from the human species. Angiopoi... |
 |
Hamster ELISA Kits | Hamster ELISA Kits A hamster is also known as suslik, which is an animal domesticated by wild animals and entered the laboratory. There are 8 kind... |
 |
BlueGene Biotech Canine Caspase 9 ELISA kit | E08C0096 Canine Caspase 9 ELISA kit Canine Caspase 9 ELISA kitis suitable for the detection of samples from Canine species. Caspase 9 can also b... |
 |
Human ELISA Kits | Our more than 8000 human visfatin elisa kitare intended to advance your research to study human disease covering several research areas (like cance... |
Same products
 |
Chlorine dioxide powder | 卖方: Shanghai Shicun Industrial Co., Ltd. | Chlorine dioxide powder Chemical formula: ClO2Molecular weight: 67.452CAS:10049-04-4EINECS:233-1... |
 |
Sodium polyacrylate | 卖方: Shanghai Shicun Industrial Co., Ltd. | Sodium polyacrylate What is Sodium Polyacrylate? Sodium polyacrylate, chemical formula(C3H3NaO2... |
 |
Thermal conductive silicone | 卖方: Shanghai Shicun Industrial Co., Ltd. | Thermal conductive silicone Thermal conductive silicone is a high-performance material designed ... |
 |
Palm oil fatty acids | 卖方: Shanghai Shicun Industrial Co., Ltd. | Palm oil fatty acids As a leadingTrusted Palm Oil Fatty Acids Supplier, we offer premium-gradeFa... |
 |
Peptide Technology Cooperation & Transfer | 卖方: PeptiOrigin Biotechnology Co., Ltd. | Peptide Technology Cooperation & Transfer Driving Innovation Through Strategic Partnerships ... |
