Boc-L-Pyroglutamic Acid Ethyl Ester

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1. Basic Information
Product Name: Boc-L-Pyroglutamic Acid Ethyl Ester / Boc-Pyr-Oet
Chemical Formula: C7H11NO3
Molecular Weight: 157.17
CAS Registry Number: 7149-65-7
EINECS Number: 230-480-9
2. Physical Parameters
Melting Point: 54-56°C
Specific Rotation: -3.5º (c=5, water)
Boiling Point: 176°C at 12 mm Hg (lit.)
Density: 1.2483 (rough estimate)
Refractive Index: 1.4310 (estimate)
Flash Point: >230°F
3. Chemical Properties and Stability
Boc-L-Pyroglutamic Acid Ethyl Ester / Boc-Pyr-Oet should be kept away from strong oxidizing agents and
strong bases, as chemical reactions may occur.
For storage, it should be sealed and placed in a cool, dry place, with a recommended storage temperature
of 4°C.
4. Uses and Applications
Boc-L-Pyroglutamic Acid Ethyl Ester / Boc-Pyr-Oet is a non-natural amino acid derivative, used for protein
modification in bacteria, yeast, and mammalian cells.
It has applications in fundamental research, drug development, and biotechnology, particularly in areas
such as protein structure change detection, drug conjugation, and biosensors.
Boc-L-Pyroglutamic Acid Ethyl Ester / Boc-Pyr-Oet can also serve as an active pharmaceutical molecule
and an intermediate in organic synthesis, such as in the synthesis of bioactive molecules like HIV integrase
inhibitors.
5. Synthesis Method
Boc-L-Pyroglutamic Acid Ethyl Ester / Boc-Pyr-Oet can be synthesized using the following method:
·Add L-glutamic acid, p-toluenesulfonic acid monohydrate, and ethanol to a flask and stir the mixture at
room temperature overnight.
·After the reaction is complete, evaporate the solvent under reduced pressure and dissolve the residue in
EtOAc.
·Stir the solution with potassium carbonate and filter, then dry the organic layer with MgSO4.
·Concentrate the organic phase under vacuum to obtain L-glutamic acid ethyl ester.
Boc-L-Pyroglutamic Acid Ethyl Ester / Boc-Pyr-Oet is a chemical compound with multiple applications and
has promising prospects in fields like drug development and biotechnology.

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