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Due to glycyl-glycine's amphoteric nature (containing both amino and carboxyl groups), glycyl-glycine acts as a physiological buffer in biological systems. It maintains pH stability in the range of 7.5–8.5, which is critical for: Stabilizing enzyme activity and protein structure, as many biological reactions are pH-dependent. Supporting cellular processes in extracellular fluids or intracellular compartments where pH fluctuations could disrupt function.

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