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BlueGene Biotech Canine Caspase 3 ELISA kit

E08C0549 Canine Caspase 3 ELISAkit

Canine Caspase 3 ELISA kitis suitable for the detection of samples from Canine species. Caspase 3 can also be called CPP32,CASP3B,SCA1,Apoptain, CASP3.

Specifications of Canine Caspase 3 ELISA kit

Product Information

E08C0549

Product Name

Canine Caspase 3 ELISA kit

Species

Canine

Product Size

48 Tests / 96 Tests

Concentration

Sensitivity

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C

Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

1 vial

STANDARD C (0.5mL)

1 ng/mL

1 vial

STANDARD D (0.5mL)

1 vial

STANDARD E (0.5mL)

5 ng/mL

1 vial

STANDARD F (0.5mL)

10 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial

Principle of the Assay

CASP3 ELISAkit applies the competitive enzyme immunoassay technique utilizing an anti-CASP3 antibody and an CASP3-HRP conjugate. The assay sample and buffer are incubated together with CASP3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CASP3 concentration since CASP3 from samples and CASP3-HRP conjugate compete for the anti-CASP3 antibody binding site. Since the number of sites is limited, as more sites are occupied by CASP3 from the sample, fewer sites are left to bind CASP3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CASP3 concentration in each sample is interpolated from this standard curve.

Quality Control on Canine Caspase 3 InsulinELISAkit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

88-102%

Linearity

Diluent Ratio

Range %

1:02

86-103

1:04

83-108

1:08

81-107

Specificity/Cross-reactivity

No significant cross-reactivity or interference between CASP3 and analogues was observed.

ShanghaiBluegene Biotechhas been committed to R&D of ELISA kits, making our ELISA kits more convenient for researchers to use on the premise of guaranteeing the quality, thus assisting scientific research for our customers. So far, we have received 1200 published papers from researchers with a total of about 500 kinds of ELISA Kits that have been cited. Bluegene ELISA kit has the advantages of high specificity, high sensitivity, and high stability.

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