BlueGene Biotech Canine Caspase 3 ELISA kit
E08C0549 Canine Caspase 3 ELISAkit
Canine Caspase 3 ELISA kitis suitable for the detection of samples from Canine species. Caspase 3 can also be called CPP32,CASP3B,SCA1,Apoptain, CASP3.
Specifications of Canine Caspase 3 ELISA kit
|
Product Information |
|
|
E08C0549 |
|
|
Product Name |
Canine Caspase 3 ELISA kit |
|
Species |
Canine |
|
Product Size |
48 Tests / 96 Tests |
|
Concentration |
|
|
Sensitivity |
|
|
Principal |
Competitive ELISA |
|
Sample Volume |
100 ul |
|
Sample Type |
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
|
Assay Time |
90 minutes |
|
Platform |
Microplate Reader |
|
Conjugate |
HRP |
|
Detection Method |
Colorimetric |
|
Storage |
2-8°C |
|
Kit Components |
||
|
MATERIALS |
SPECIFICATION |
QUANTITY |
|
MICROTITER PLATE |
96 wells |
stripwell |
|
ENZYME CONJUGATE |
1 vial |
|
|
STANDARD A (0.5mL) |
0 ng/mL |
1 vial |
|
STANDARD B (0.5mL) |
1 vial |
|
|
STANDARD C (0.5mL) |
1 ng/mL |
1 vial |
|
STANDARD D (0.5mL) |
1 vial |
|
|
STANDARD E (0.5mL) |
5 ng/mL |
1 vial |
|
STANDARD F (0.5mL) |
10 ng/mL |
1 vial |
|
SUBSTRATE A |
6 mL |
1 vial |
|
SUBSTRATE B |
6 mL |
1 vial |
|
STOP SOLUTION |
6 mL |
1 vial |
|
WASH SOLUTION (100 x) |
10 mL |
1 vial |
|
BALANCE SOLUTION |
3 mL |
1 vial |
Principle of the Assay
CASP3 ELISAkit applies the competitive enzyme immunoassay technique utilizing an anti-CASP3 antibody and an CASP3-HRP conjugate. The assay sample and buffer are incubated together with CASP3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CASP3 concentration since CASP3 from samples and CASP3-HRP conjugate compete for the anti-CASP3 antibody binding site. Since the number of sites is limited, as more sites are occupied by CASP3 from the sample, fewer sites are left to bind CASP3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CASP3 concentration in each sample is interpolated from this standard curve.
Quality Control on Canine Caspase 3 InsulinELISAkit
|
Coefficient of Variance |
Intra Variation% ＜10% |
|
|
Inter Variation% ＜12% |
||
|
Recovery |
88-102% |
|
|
Linearity |
Diluent Ratio |
Range % |
|
1:02 |
86-103 |
|
|
1:04 |
83-108 |
|
|
1:08 |
81-107 |
|
|
Specificity/Cross-reactivity |
No significant cross-reactivity or interference between CASP3 and analogues was observed. |
|
ShanghaiBluegene Biotechhas been committed to R&D of ELISA kits, making our ELISA kits more convenient for researchers to use on the premise of guaranteeing the quality, thus assisting scientific research for our customers. So far, we have received 1200 published papers from researchers with a total of about 500 kinds of ELISA Kits that have been cited. Bluegene ELISA kit has the advantages of high specificity, high sensitivity, and high stability.
If you want to know more kinds of bovine igg elisa kits, please visit our website.
Send product request
Other supplier products
 |
BlueGene Biotech Canine BCL2 Associated X Protein ELISA kit | ShanghaiBlueGene Biotech Canine BCL2 Associated X Protein ELISA kit E08B0032 Canine BCL2 Associated X Protein ELISA kit The Canine BCL2 Associ... |
 |
BlueGene Biotech Bovine Cluster Of Differentiation 8 ELISA kit | E11C0007 Bovine Cluster Of Differentiation 8 Bluegene ELISA kit Bovine Cluster of Differentiation 8 insulin ELISAkit is suitable for the detecti... |
 |
BlueGene Biotech Porcine Total Adiponectin ELISA kit | BlueGene Biotech Porcine Total Adiponectin ELISAkit Adiponectin can also be called 30 kDa adipocyte complement related protein, 30 kDa adipocyte ... |
 |
BlueGene Biotech Canine β Cell Leukemia/Lymphoma 2 ELISA kit | E08B0033 Canine β Cell Leukemia/Lymphoma 2 ELISA kit Canine β Cell Leukemia/Lymphoma 2 ELISA kit is suitable for the detection of samp... |
 |
BlueGene Biotech Sheep Anti Interleukin 10 ELISA kit | Specifications of Sheep Anti Interleukin 10 ELISAkit Product Information E14A0081 Product Name Sheep Anti Inter... |
Same products
 |
BlueGene Biotech Sheep Cluster of Differentiation 4 ELISA kit | Seller: Bluegene Biotech | E14C0004 Sheep Cluster of Differentiation 4 ELISA kit Sheep Cluster of Differentiation 4 ELISA... |
|
|
BlueGene Biotech Sheep Anti Interleukin 10 ELISA kit | Seller: Bluegene Biotech | Specifications of Sheep Anti Interleukin 10 ELISAkit Product Information E1... |
 |
BlueGene Biotech Chicken Total Adiponectin ELISA kit | Seller: Bluegene Biotech | E12A0125 Chicken Total Adiponectin ELISA kit Chicken Total Adiponectin ELISA kit (a knid of ch... |
 |
BlueGene Biotech Bovine Decorin ELISA kit | Seller: Bluegene Biotech | E11D0145 Bovine Decorin ELISA kit Bovine Decorin ELISAkit is suitable for the detection of sam... |
 |
BlueGene Biotech Bovine C Type Natriuretic Peptide ELISA kit | Seller: Bluegene Biotech | E11C0339 Bovine C Type Natriuretic PeptideELISA kit Bovine C Type Natriuretic Peptide ELISAkit... |
