BlueGene Biotech Sheep Anti Interleukin 10 ELISA kit
Specifications of Sheep Anti Interleukin 10 ELISAkit
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Product Information |
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E14A0081 |
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Product Name |
Sheep Anti Interleukin 10 ELISA kit |
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Species |
Sheep |
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Product Size |
48 Tests / 96 Tests |
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Concentration |
25-500 ng/ml |
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Sensitivity |
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Principal |
Competitive ELISA |
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Sample Volume |
100 ul |
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Sample Type |
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
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Assay Time |
90 minutes |
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Platform |
Microplate Reader |
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Conjugate |
HRP |
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Detection Method |
Colorimetric |
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Storage |
2-8°C |
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Kit Components |
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MATERIALS |
SPECIFICATION |
QUANTITY |
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MICROTITER PLATE |
96 wells |
stripwell |
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ENZYME CONJUGATE |
1 vial |
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STANDARD A (0.5mL) |
0 ng/mL |
1 vial |
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STANDARD B (0.5mL) |
25 ng/mL |
1 vial |
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STANDARD C (0.5mL) |
50 ng/mL |
1 vial |
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STANDARD D (0.5mL) |
100 ng/mL |
1 vial |
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STANDARD E (0.5mL) |
250 ng/mL |
1 vial |
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STANDARD F (0.5mL) |
500 ng/mL |
1 vial |
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SUBSTRATE A |
6 mL |
1 vial |
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SUBSTRATE B |
6 mL |
1 vial |
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STOP SOLUTION |
6 mL |
1 vial |
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WASH SOLUTION (100 x) |
10 mL |
1 vial |
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BALANCE SOLUTION |
3 mL |
1 vial |
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Principle of the Assay |
Anti-il 10 elisaapplies the competitive enzyme immunoassay technique utilizing IL 10 antigen and an Anti-IL 10-HRP conjugate. The assay sample and buffer are incubated together with Anti-IL 10-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Anti-IL 10 concentration since Anti-IL 10 from samples and Anti-IL 10-HRP conjugate compete for the IL 10 antigen binding site. Since the number of sites is limited, as more sites are occupied by Anti-IL 10 from the sample, fewer sites are left to bind Anti-IL 10-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Anti-IL 10 concentration in each sample is interpolated from this standard curve.
Quality Control on Sheep Anti Interleukin 10 ELISA kit
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Coefficient of Variance |
Intra Variation% ＜10% |
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Inter Variation% ＜12% |
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Recovery |
87-118.37% |
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Linearity |
Diluent Ratio |
Range % |
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1:2 |
98-117 |
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1:4 |
93-106 |
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1:8 |
91-105 |
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Specificity/Cross-reactivity |
No significant cross-reactivity or interference between Anti IL 10 and analogues was observed. |
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Bluegene Biotech has been committed to R&D of ELISA kits, making our ELISA kits more convenient for researchers to use on the premise of guaranteeing the quality, thus assisting scientific research for our customers. So far, we have received 1200 published papers from researchers with a total of about 500 kinds of ELISA Kits that have been cited. Bluegene ELISA kithas the advantages of high specificity, high sensitivity, and high stability.
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