BlueGene Biotech Canine Endothelin 1 ELISA kit
E08E0040 Canine Endothelin 1 ELISAkit
Canine Endothelin 1 ELISA kitis suitable for the detection of samples from Canine species. Endothelin 1 can also be called Endothelin-1, Big endothelin 1, EDN1 protein, Endothelin1, ET1, PPET1, Preproendothelin 1.
Specifications of Canine EndothelinELISAkit
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Product Information |
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E08E0040 |
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Product Name |
Canine Endothelin 1 ELISA kit |
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Species |
Canine |
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Product Size |
48 Tests / 96 Tests |
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Concentration |
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Sensitivity |
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Principal |
Competitive ELISA |
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Sample Volume |
100 ul |
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Sample Type |
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
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Assay Time |
90 minutes |
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Platform |
Microplate Reader |
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Conjugate |
HRP |
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Detection Method |
Colorimetric |
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Storage |
2-8°C |
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Kit Components |
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MATERIALS |
SPECIFICATION |
QUANTITY |
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MICROTITER PLATE |
96 wells |
stripwell |
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ENZYME CONJUGATE |
1 vial |
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STANDARD A (0.5mL) |
0 ng/mL |
1 vial |
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STANDARD B (0.5mL) |
1 vial |
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STANDARD C (0.5mL) |
1 ng/mL |
1 vial |
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STANDARD D (0.5mL) |
1 vial |
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STANDARD E (0.5mL) |
5 ng/mL |
1 vial |
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STANDARD F (0.5mL) |
10 ng/mL |
1 vial |
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SUBSTRATE A |
6 mL |
1 vial |
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SUBSTRATE B |
6 mL |
1 vial |
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STOP SOLUTION |
6 mL |
1 vial |
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WASH SOLUTION (100 x) |
10 mL |
1 vial |
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BALANCE SOLUTION |
3 mL |
1 vial |
Principle of the Assay
BigEndothelin 1 ELISAkit applies the competitive enzyme immunoassay technique utilizing an anti-EDN1 antibody and an EDN1-HRP conjugate. The assay sample and buffer are incubated together with EDN1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the EDN1 concentration since EDN1 from samples and EDN1-HRP conjugate compete for the anti-EDN1 antibody binding site. Since the number of sites is limited, as more sites are occupied by EDN1 from the sample, fewer sites are left to bind EDN1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The EDN1 concentration in each sample is interpolated from this standard curve.
Quality Control on Canine Endothelin 1 ELISA kit
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Coefficient of Variance |
Intra Variation% ＜10% |
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Inter Variation% ＜12% |
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Recovery |
89-108% |
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Linearity |
Diluent Ratio |
Range % |
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1:02 |
86-105 |
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1:04 |
85-110 |
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1:08 |
82-112 |
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Specificity/Cross-reactivity |
No significant cross-reactivity or interference between EDN1 and analogues was observed. |
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Bluegene Biotechhas been committed to R&D of ELISA kits, making our ELISA kits more convenient for researchers to use on the premise of guaranteeing the quality, thus assisting scientific research for our customers. So far, we have received 1200 published papers from researchers with a total of about 500 kinds of ELISA Kits that have been cited. Bluegene ELISA kit has the advantages of high specificity, high sensitivity, and high stability.
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