Heat Resistance of Nisin

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Heat Resistance of Nisin Nisin possesses excellent heat stability, a critical advantage for high-temperature processed foods. It retains over 80% of activity after autoclaving at 121℃ for 15–30 minutes, and over 90% after pasteurization (72–85℃ for 15–30 seconds). This stability is attributed to its unique lanthionine and methyllanthionine cross-links, which enhance peptide chain rigidity. However, prolonged exposure to ultra-high temperatures (>130℃) or combination with reducing substances (e.g., mercaptoethanol) can disrupt its cross-linked structure, resulting in activity degradation. In practical applications, Nisin is often added after high-temperature processing or during the cooling phase to maximize activity retention, especially in canned foods and sterilized dairy products.

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