Aflatoxins B1 ELISA test kit
Aflatoxins B1 (AFB1) ELISA Test Kit
Catalog No. LSY-10028
1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Aflatoxins B1. The coupling antigen is pre-coated on the micro-well stripes. The Aflatoxins B1 in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti- Aflatoxins B1 antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Aflatoxins B1 in the sample. This value is compared to the standard curve and the Aflatoxins B1 residues is subsequently obtained.2. Technical specifications
Sensitivity: 0.03 ppb
Incubator temperature: 25℃
Incubator time: 30min～15min
Detection limit:
Milk (Method 1)…………………………………………………….……...0.15ppb
Milk (Method 2)…………………………………………….....…………....0.3ppb
Tissue……………………………………………………………………...0.72ppb
Feed ……………………………………………………………………..…0.9ppb
Urine………………………………………………………………..….….0.06ppb
Cross-reaction rate:
Aflatoxins B1… ……..100%
Recovery rate:
Milk……………………………………………………………………….… 90±23%
Tissue…………………………………………………………………..…. 85±18%
Feed,urine……………………………………………….……………….. 85±21%3. Components
1) Micro-well strips: 12 strips with 8 removable wells each
2) 6× standard solution (1 mL each): 0 ppb, 0.03 ppb, 0.06 ppb, 0.12 ppb, 0.24 ppb ,0.48 ppb
3) Enzyme conjugate (7 mL) red cap
4) Antibody working solution (7 mL) blue cap
5) Substrate A solution (7 mL) white cap
6) Substrate B solution (7 mL) black cap
7) Stop solution (7 mL) yellow cap
8) 20× concentrated washing buffer (40 mL) white cap
9) 2× concentrated redissolving solution (50 mL) transparent cap
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