Rat Epinephrine/Adrenaline ELISA kit

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E02A0007 Rat Epinephrine/Adrenaline ELISA kit

Rat Adrenaline/Epinephrine ELISA kitis suitable for the detection of samples from Rat species. Epinephrine/Adrenaline can also be called ADR, or EPI.

Specifications of Rat Adrenaline/Epinephrine ELISAkit

Principle of the Assay

EPI ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-EPI antibody and an EPI-HRP conjugate. The assay sample and buffer are incubated together with EPI-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the EPI concentration since EPI from samples and EPI-HRP conjugate compete for the anti-EPI antibody binding site. Since the number of sites is limited, as more sites are occupied by EPI from the sample, fewer sites are left to bind EPI-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The EPI concentration in each sample is interpolated from this standard curve.

Citations of Rat Epinephrine/Adrenaline ELISA kit

E02A0007 has been referenced in the below publications:

Role of plateau hypoxia hypothermia complex damage to the vascular endothelial cells and the protective effect.

The mechanism research of neurons injury in the basolateral of amygdala induced by stress.

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