TAPS BUFFER CAS NO.29915-38-6
Trimethylolaminopropanesulfonic acid, or TAPS for short, is a white crystalline organic compound that emits a volatile and irritating odor when placed in the air for a long time at room temperature. This TAPS buffer is usually configured as a biological buffer with a sodium hydroxide lye instrument to control the pH range in biochemical reactions.
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CAS No. |
29915 38 6 |
Melting point |
230-235 °C (dec.) |
Solubility |
H2O: 1 M at 20 °C, clear, colorless |
|
MF |
C7H17NO6S |
Flash Point |
110 °C |
Acidity factor (pKa) |
|
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MW |
Density |
Acid-base indicator colour change ph range |
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Appearance |
White crystalline powder |
Packaging |
25kg/barrel |
Storage |
Store sealed at room temperature |
TAPS Buffer Details
TAPS, the full name of N-tris (hydroxymethyl) methyl-3-aminopropanesulfonic acid, CAS number: 29915-38-6, pH buffer range 7.7-9.1, soluble in water (25g/50ml). It is a white crystalline powder of zwitterionic buffer widely used in the fields of biochemistry and molecular biology. It emits volatile and irritating odor when placed in the air at room temperature. It is often used as a biological buffer. It has good water solubility and usually the concentration is 25g/50ml, which is widely used in the fields of biochemistry and molecular biology.
In biochemical research experiments such as biochemical diagnostic kits, DNA/RNA extraction and PCR diagnosis, biological buffer TAPS is usually used to control the pH value of the reaction system, and TAPS buffer is also used in some high-standard cell culture experiments agent. There are two types of TAPS reagents on the market, one is a white crystalline powder, and the other is a ready-to-use buffer solution formulated for a specific pH range and different concentrations.
Desheng's Advantages in TAPS Buffer
The stock can be provided according to the customer's demand. If the demand is not large, the TAPS ready-to-use buffer can be prepared according to the customer's demand. The specifications are usually 200mL, 500mL, 1L, etc. After arrival, it is very convenient to use it out of the box, but the price is relatively high.
If the demand is large or long-term use is required, we have TAPS buffer powder. This is more conducive to long-term storage. It can be prepared at any time when needed, and different concentrations and pH values can be adjusted as needed, making it more flexible to use. Moreover, TAPS buffer powder can save a lot of price compared with ready-to-use buffer.
TAPS buffer is of high purity and good quality. We specially equip professionals to test the various indicators of the product. The product has the characteristics of high purity and small-batch difference;
White crystal, high purity (more than 99%), good solubility, absorbance<0.05, different from light blue or brown products on the market.
Have a professional foreign trade team and rich export experience.
Application Of Biological Buffer TAPS
TAPS is a commonly used buffer system in DNA sieving system, and can also be used as a buffer component of RNA samples. It is suitable for electron transport and phosphorylation studies of chloroplast thin-layer preparation.
Protects oxyhemoglobin from oxidizing to methemoglobin during freeze-drying
It can be used as a background electrolyte for protein micro-analysis by capillary zone electrophoresis.
It can be used as an important PH stabilizer, generally mixed with weak acid and its conjugate base to obtain appropriate PH value.
Usually used in some biochemical diagnostic kits, PCR diagnostic kits and DNA/RNA extraction kits.
Trimethylolmethylaminopropanesulfonic Acid (TAPS) Instructions for Use:
Completely dissolve the weighed TAPS reagent powder with deionized water. Be careful not to add too much water at the beginning of the dissolution, and ensure that it is completely dissolved. Finally, continue to add water to the volume;
Pay attention to the temperature when setting the volume. Due to the exothermic or endothermic temperature change during dissolution, the volume should be fixed when the temperature is close to room temperature;
After the configuration, seal the cap of the volumetric flask, which can be kept for future use. Mark the TAPS solution, and mark the concentration configuration date and other information.
TAPS Buffer Notes
The product can be stored at room temperature. If strong light cannot be seen, a container with better shading should be used. The place should be moisture-proof and dry;
If you can't use it all at once, it is recommended to divide it into the amount you need each time, and seal the bottle well, so as to avoid moisture absorption or product quality problems during use.
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