Chloramphenicol(CAP) ELISA Test Kit
Chloramphenicol ELISA test Kit Catalog No. LSY-10007 1. Principle This test kit is based on the competitive enzyme immunoassay for the detection of Chloramphenicol in the sample. The coupling antigen is pre-coated on the micro-well stripes. The Chloramphenicol in the sample and the coupling antigen pre-coated on the micro-well stripes compete for the anti-Chloramphenicol antibody. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Chloramphenicol in it. This value is compared to the standard curve and the Chloramphenicol concentration is subsequently obtained. 2. Technical specifications Sensitivity: 0.05 ppb Detection limit: Intestine 0.1 ppb Honey 0.1 ppb Milk 0.05 ppb Urine, serum 0.1 ppb Tissue, liver 0.025 ppb Feed…………………………………………………………………………0.15 ppb Recovery rate Tissue, liver 80±10% Honey, intestine 70±10% Milk, feed 85±15% Urine, serum…………………………………………………………………70±10% Cross-reaction rate: Chloramphenicol 100% Thiamphenicol < 0.1% Florfeniol < 0.1% 3. Components 1） Micro-well strips: 12 strips with 8 removable wells each 2） 6× standard solution (1 mL each): 0 ppb, 0.05 ppb, 0.15 ppb, 0.45 ppb, 1.35 ppb and 4.05 ppb 3） Enzyme conjugate (12 mL) red cap 4） Concentrated antibody working solution (1 mL) blue cap 5） Substrate A solution (7 mL) white cap 6） Substrate B solution (7 mL) black cap 7） Stop solution (7 mL) yellow cap 8） 20× concentrated washing buffer (40 mL) white cap 9） 2× concentrated redissolving solution (50 mL) transparent cap
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