Malachite green ELISA kit
Malachite green ELISA kit
Catalog No. LSY-10027
This test kit is based on the competitive enzyme immunoassay. The coupling antigen is pre-coated on the micro-well stripes. The Malachite green in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Malachite green antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Malachite green in it. This value is compared to the standard curve and the content of Malachite green residues is subsequently obtained.
Sensitivity: 0.05 ppb
Incubator temperature: 25℃
Incubator time: 30min～30min～15min
Detection limit
Aquatic products e.g. Shrimp, Fish................................................... 0.5 ppb
Water............................................................................................... 0.1 ppb
Recovery rate
Shrimp, Fish, water...................................................................... 80%--110%
Cross-reaction rate
Malachite green................................................................................... 100%
Leucomalachite green........................................................................... 0.1%
Gentian violet....................................................................................... 95%
Leucocrystal violet................................................................................ 0.1%
1） Micro-well strips: 12 strips with 8 removable wells each
2） 6× standard solution (1 mL each, white cap): 0 ppb, 0.05 ppb, 0.15 ppb,
3） Enzyme conjugate (12 mL)............................................................. red cap
4） Antibody working solution (7 mL).................................................. blue cap
5） Substrate A solution (7 mL).......................................................... white cap
6） Substrate B solution (7 mL)……………………………………………. black cap
7） Stop solution (7 mL).................................................................. yellow cap
8） 20× concentrated washing buffer (40 mL)..................................... white cap
9） Oxidant (3 mL)............................................................................ black cap
10) 2× concentrated redissolving solution (50 mL).................... transparent cap
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