Malachite green ELISA kit

Malachite green ELISA kit

Catalog No. LSY-10027

This test kit is based on the competitive enzyme immunoassay. The coupling antigen is pre-coated on the micro-well stripes. The Malachite green in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Malachite green antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Malachite green in it. This value is compared to the standard curve and the content of Malachite green residues is subsequently obtained.

Sensitivity: 0.05 ppb

Incubator temperature: 25℃

Incubator time: 30min～30min～15min

Detection limit

Aquatic products e.g. Shrimp, Fish................................................... 0.5 ppb

Water............................................................................................... 0.1 ppb

Recovery rate

Shrimp, Fish, water...................................................................... 80%--110%

Cross-reaction rate

Malachite green................................................................................... 100%

Leucomalachite green........................................................................... 0.1%

Gentian violet....................................................................................... 95%

Leucocrystal violet................................................................................ 0.1%

1） Micro-well strips: 12 strips with 8 removable wells each

2） 6× standard solution (1 mL each, white cap): 0 ppb, 0.05 ppb, 0.15 ppb,

3） Enzyme conjugate (12 mL)............................................................. red cap

4） Antibody working solution (7 mL).................................................. blue cap

5） Substrate A solution (7 mL).......................................................... white cap

6） Substrate B solution (7 mL)……………………………………………. black cap

7） Stop solution (7 mL).................................................................. yellow cap

8） 20× concentrated washing buffer (40 mL)..................................... white cap

9） Oxidant (3 mL)............................................................................ black cap

10) 2× concentrated redissolving solution (50 mL).................... transparent cap

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