BIS-TRIS CAS NO.6976-37-0
Bis-Tris is a very versatile zwitterionic biological buffer commonly used in biological and biochemical research. Bis-Tris is named according to its structure. Unlike most other available biological buffers, Bis-Tris' has functional groups associated with two distinct structural families: the bis(2-hydroxyethyl)amine family and the Tris family.
Bis-tris in Detail
Bis-Tris full name is bis (2-hydroxyethyl) amino (trimethylol) methane, CAS number: 6976-37-0, is an inert zwitterionic buffer, the effective pH buffer range is 5.8-7.2, pKa =6.5 (25°C), the structure is similar to Tris, hence the name. Bis-Tris has only a slight change in its dissociation constant with the change of temperature. It can ionize a small amount of hydrogen ions and is weakly acidic, so it is suitable for weakly acidic buffer systems.
Advantages of Using Bis-tris Gels for Protein Electrophoresis:
The gel runs at neutral pH, which minimizes protein denaturation and helps improve protein stability during electrophoresis, which in turn helps to improve band clarity and resolution;
The electrophoresis time is about 40% shorter than that of standard Tris-glycine;
The casted gel can be stored at 4°C for up to 4 weeks and is compatible with standard staining and transfer methods;
Premixed acrylamide buffer improves reproducibility while saving time;
Flexibility: Dilute the separation solution with deionized water to make its gel percentage reach any value between 8% and 15%;
The rapid glue making solution supports the simultaneous polymerization of lamination glue and separation glue, which can further save time.
Advantages of Desheng in Bis-tris:
Reliable quality, professional production base, using imported production equipment to ensure that Bis-tris can meet customer requirements in all parameters;
The after-sales service is good. After purchasing the raw materials, the customer may encounter various problems and needs to be guided by a special person. This requires the selected raw material supplier to provide the corresponding after-sales service. In this regard, Desheng is very professional;
Good reputation in the industry, which can better reflect the comprehensive strength of an enterprise. As a bis tris manufacturer, Desheng has a good reputation in the market and has been affirmed by many companies;
The manufacturer provides stable supply. Desheng has a high daily output and sufficient inventory reserves. Even if there is a large demand, Desheng can deliver the money quickly.
For agarose gel electrophoresis;
It can protect its stability in the separation of hemoglobin;
It can be used as an effective buffer for the analysis of muscle enzyme activity.
Bis-tris Notes
Forms strong complexes with Pb2+ and Cu2+, a weak interaction with other common metals;
When used with BCA detection, it will affect the use effect;
To avoid affecting protein kinetics, it is not recommended to use with FAB.
Bis-Tris forms complexes with heavy metals such as lead and copper.
Want to know the details of cas 6976 37 0and 82611 88 9, contact us.
在线联系供应商
Other supplier products
 |
PEP PHOSPHOENOLPYRUVATE BUFFER CAS NO.35556-70-8 | Phosphoenol pyruvate(PEP) has a variety of effects, that is, enzyme substrate products, can also be used as a biological buffer, and because of its... |
 |
TRISODIUM CITRATE DIHYDRATE CAS NO.6132-04-3 | Sodium citrate is widely used. Desheng provides analytically pure sodium citrate which is specially used as blood anticoagulant in blood collection... |
 |
TES BUFFER CAS NO.7365-44-8 | The name of TES is n-trimethylol-methyl-2 amino-ethanesulfonic acid. TES powder can not only be prepared into biological buffer, but also is a very... |
 |
TAPS BUFFER CAS NO.29915-38-6TAPS BUFFER CAS NO.29915-38-6 | Trimethylolaminopropanesulfonic acid, or TAPS for short, is a white crystalline organic compound that emits a volatile and irritating odor when pla... |
 |
TRIS HYDROCHLORIDE CAS NO.1185-53-1 | Tris hydrochloride raw material, or tri(hydroxymethyl)aminomethane hydrochloride, is a very common biological buffer, widely used in nucleic acids,... |
Same products
 |
C500-1.7 Series High Flow Multi-Stage Centrifugal Blower /Centrifugal Blower | 卖方: Greentech International (Zhangqiu) Co., Ltd | Greentech International (Zhangqiu) Co., Ltdis the professional blower supplier.Multistage Centrif... |
 |
Common Rail injector control valve F00VC01303 | 卖方: China Lutong Part Plant | Common Rail injector control valve F00VC01303 Tina#Stainless Steel Cylindrical Pin##Denso Common... |
 |
Common Rail injector control valve F00VC01200 | 卖方: China Lutong Part Plant | Common Rail injector control valve F00VC01200 Tina#Stainless Steel Cylindrical Pin##Denso Common... |
 |
Injector Control Valve Plate 24# | 卖方: China Lutong Part Plant | Injector Control Valve Plate 24# Tina#Stainless Steel Cylindrical Pin##Denso Common Rail Injecto... |
 |
Injector Control Valve Plate 12# | 卖方: China Lutong Part Plant | Injector Control Valve Plate 12# Tina#Stainless Steel Cylindrical Pin##Denso Common Rail Injecto... |
