Keratinase
Protease catalyzes peptide bond and hydrolyzes protein into peptone, hydrazone, polypeptide and amino acid. As a kind of alkaline serine protease, keratinase enzymenot only has the function of normal alkaline protease, but also can break down disulfide bond and disintegrate keratin.
Specification of keratinase products
|
Name |
Keratinase |
Product Series Name |
NutriGen Keratinase |
|
Activity |
U/g |
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|
Strain |
Parengyodontium album |
EC code |
EC 3.4.99.11 |
|
Form |
Powder,Liquid |
Carrier |
Starch |
|
Color |
White, Light yellow |
Loss on drying |
≤ 10% |
|
Working pH |
Optimum pH |
11 |
|
|
Working T |
30℃-70℃ |
Optimum T |
60℃ |
|
Particle size |
80% pass 40 mesh |
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|
Package |
25kg /bag |
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|
Storage |
Stored 6 months in dark, cool and dry conditions |
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|
Caution |
Avoid direct sunlight, high temperature |
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Definition and Dosage of Activity Keratinase
One unit (U) of protease activity is defined as the amount of enzyme chinawhich liberates 1 μg tyrosine per minute from substrate casein at 40℃ and pH 10.5 under the conditions of the test.
Recommended dosage is 50-100g/t with 50000 U/g in complete feed.
Functions of Keratinase
- ’t do.
Application of Keratinase
|
Nutrient |
Poultry |
|
|
Value |
Amount provided in the diet |
|
|
Metabolizable energy（Mcal/kg） |
600 |
|
|
Crude protein, % |
5400 |
|
|
Lysine, % |
150 |
|
|
Methionine, % |
180 |
|
|
Cystine, % |
150 |
|
|
Threonine, % |
210 |
|
|
Tryptophane, % |
90 |
|
|
Isoleucine , % |
150 |
|
|
Arginine, % |
285 |
|
|
Valine, % |
255 |
|
Experimental animal: 840 one-day-old AA broilers, 6×35 broilers/treatment.
Experimental design:
|
Item |
Diet |
|
PC |
Basal diet |
|
NC |
Basal diet-0.5% crude protein |
|
Group I |
NC+125g/t competitor |
|
Group II |
NC+100g/t Keratinase(30000U/g) |
Experimental cycle: 42 days
Results:
Table2 Effect of each treatment on the growth performance of AA broilers (g, %)
|
Items |
PC |
NC |
Group I |
Group II |
|
Initial weight,g |
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|
1-21days |
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|
21stAG,g |
|
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|
ADFI,g |
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|
ADG,g |
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|
F/G |
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|
Death rate,% |
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|
22-42days |
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|
22ndAG,g |
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|
42ndAG,g |
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|
ADFI,g |
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|
ADG,g |
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|
F/G |
||||
|
Death rate,% |
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|
1-42days |
||||
|
ADFI,g |
||||
|
ADG,g |
||||
|
F/G |
||||
|
Death rate,% |
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Note: In the same row, values with different small letter superscripts mean significant difference(P<0.05).
Diagram1 Effect of keratinase on the nutrient digestibility of broilers
Table3 Effect of keratinase on the amino acid digestibility of broilers （%）
|
Items |
PC |
NC |
Group I |
Group II |
|
Asp |
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|
Thr |
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|
Ser |
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|
Glu |
|
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|
Gly |
|
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|
Ala |
|
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Val |
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Met |
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|
Ile |
|
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Leu |
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|
Tyr |
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|
Phe |
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|
His |
|
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|
Lys |
|
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|
Arg |
|
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|
Pro |
|
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|
TAA |
|
Conclusion of Keratinase
On the basis of these results, it is concluded that the supplement of keratinase into a low CP diet was as effective as normal level in broiler. As shown in diagram 1, keratinase improve the digestibility of dry matter, crude protein, crude fat, general energy and apparent metabolic energy. And in table 4, keratinase has a positive effect on the amino acids digestibility. In this experiment, it is appeared that keratinase improve the growth performance of broiler by increase average diary gain and feed conversion rate.
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