Keratinase

Protease catalyzes peptide bond and hydrolyzes protein into peptone, hydrazone, polypeptide and amino acid. As a kind of alkaline serine protease, keratinase enzymenot only has the function of normal alkaline protease, but also can break down disulfide bond and disintegrate keratin.

Specification of keratinase products

Name	Keratinase	Product Series Name	NutriGen Keratinase
Activity	U/g
Strain	Parengyodontium album	EC code	EC 3.4.99.11
Form	Powder,Liquid	Carrier	Starch
Color	White, Light yellow	Loss on drying	≤ 10%
Working pH		Optimum pH	11
Working T	30℃-70℃	Optimum T	60℃
Particle size	80% pass 40 mesh
Package	25kg /bag
Storage	Stored 6 months in dark, cool and dry conditions
Caution	Avoid direct sunlight, high temperature

Definition and Dosage of Activity Keratinase

One unit (U) of protease activity is defined as the amount of enzyme chinawhich liberates 1 μg tyrosine per minute from substrate casein at 40℃ and pH 10.5 under the conditions of the test.

Recommended dosage is 50-100g/t with 50000 U/g in complete feed.

Functions of Keratinase

3. ’t do.

Application of Keratinase

Nutrient	Poultry
	Value	Amount provided in the diet
Metabolizable energy（Mcal/kg）	600
Crude protein, %	5400
Lysine, %	150
Methionine, %	180
Cystine, %	150
Threonine, %	210
Tryptophane, %	90
Isoleucine , %	150
Arginine, %	285
Valine, %	255

Experimental animal: 840 one-day-old AA broilers, 6×35 broilers/treatment.

Experimental design:

Item	Diet
PC	Basal diet
NC	Basal diet-0.5% crude protein
Group I	NC+125g/t competitor
Group II	NC+100g/t Keratinase(30000U/g)

Experimental cycle: 42 days

Results:

Table2 Effect of each treatment on the growth performance of AA broilers (g, %)

Items	PC	NC	Group I	Group II
Initial weight,g
1-21days
21stAG,g	ab±10.08 b±8.22 ab±25.72 ab±10.53
ADFI,g
ADG,g
F/G
Death rate,%
22-42days
22ndAG,g
42ndAG,g
ADFI,g
ADG,g
F/G
Death rate,%
1-42days
ADFI,g
ADG,g
F/G
Death rate,%

Note: In the same row, values with different small letter superscripts mean significant difference(P<0.05).

Diagram1 Effect of keratinase on the nutrient digestibility of broilers

Table3 Effect of keratinase on the amino acid digestibility of broilers （%）

Items	PC	NC	Group I	Group II
Asp
Thr
Ser
Glu	ab b a
Gly	ab b b a
Ala	b b b
Val
Met
Ile	b ab ab
Leu
Tyr
Phe
His	ab b ab
Lys	ab b a
Arg	ab b ab
Pro	b ab b
TAA	ab b a

Conclusion of Keratinase

On the basis of these results, it is concluded that the supplement of keratinase into a low CP diet was as effective as normal level in broiler. As shown in diagram 1, keratinase improve the digestibility of dry matter, crude protein, crude fat, general energy and apparent metabolic energy. And in table 4, keratinase has a positive effect on the amino acids digestibility. In this experiment, it is appeared that keratinase improve the growth performance of broiler by increase average diary gain and feed conversion rate.

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