Keratinase
Protease catalyzes peptide bond and hydrolyzes protein into peptone, hydrazone, polypeptide and amino acid. As a kind of alkaline serine protease, keratinase enzymenot only has the function of normal alkaline protease, but also can break down disulfide bond and disintegrate keratin.
Specification of keratinase products
Name |
Keratinase |
Product Series Name |
NutriGen Keratinase |
Activity |
U/g |
||
Strain |
Parengyodontium album |
EC code |
EC 3.4.99.11 |
Form |
Powder,Liquid |
Carrier |
Starch |
Color |
White, Light yellow |
Loss on drying |
≤ 10% |
Working pH |
Optimum pH |
11 |
|
Working T |
30℃-70℃ |
Optimum T |
60℃ |
Particle size |
80% pass 40 mesh |
||
Package |
25kg /bag |
||
Storage |
Stored 6 months in dark, cool and dry conditions |
||
Caution |
Avoid direct sunlight, high temperature |
Definition and Dosage of Activity Keratinase
One unit (U) of protease activity is defined as the amount of enzyme chinawhich liberates 1 μg tyrosine per minute from substrate casein at 40℃ and pH 10.5 under the conditions of the test.
Recommended dosage is 50-100g/t with 50000 U/g in complete feed.
Functions of Keratinase
- ’t do.
Application of Keratinase
Nutrient |
Poultry |
|
Value |
Amount provided in the diet |
|
Metabolizable energy(Mcal/kg) |
600 |
|
Crude protein, % |
5400 |
|
Lysine, % |
150 |
|
Methionine, % |
180 |
|
Cystine, % |
150 |
|
Threonine, % |
210 |
|
Tryptophane, % |
90 |
|
Isoleucine , % |
150 |
|
Arginine, % |
285 |
|
Valine, % |
255 |
Experimental animal: 840 one-day-old AA broilers, 6×35 broilers/treatment.
Experimental design:
Item |
Diet |
PC |
Basal diet |
NC |
Basal diet-0.5% crude protein |
Group I |
NC+125g/t competitor |
Group II |
NC+100g/t Keratinase(30000U/g) |
Experimental cycle: 42 days
Results:
Table2 Effect of each treatment on the growth performance of AA broilers (g, %)
Items |
PC |
NC |
Group I |
Group II |
Initial weight,g |
||||
1-21days |
||||
21stAG,g |
|
|||
ADFI,g |
||||
ADG,g |
||||
F/G |
||||
Death rate,% |
||||
22-42days |
||||
22ndAG,g |
||||
42ndAG,g |
||||
ADFI,g |
||||
ADG,g |
||||
F/G |
||||
Death rate,% |
||||
1-42days |
||||
ADFI,g |
||||
ADG,g |
||||
F/G |
||||
Death rate,% |
Note: In the same row, values with different small letter superscripts mean significant difference(P<0.05).
Diagram1 Effect of keratinase on the nutrient digestibility of broilers
Table3 Effect of keratinase on the amino acid digestibility of broilers (%)
Items |
PC |
NC |
Group I |
Group II |
Asp |
||||
Thr |
||||
Ser |
||||
Glu |
|
|||
Gly |
|
|||
Ala |
|
|||
Val |
||||
Met |
||||
Ile |
|
|||
Leu |
||||
Tyr |
||||
Phe |
||||
His |
|
|||
Lys |
|
|||
Arg |
|
|||
Pro |
|
|||
TAA |
|
Conclusion of Keratinase
On the basis of these results, it is concluded that the supplement of keratinase into a low CP diet was as effective as normal level in broiler. As shown in diagram 1, keratinase improve the digestibility of dry matter, crude protein, crude fat, general energy and apparent metabolic energy. And in table 4, keratinase has a positive effect on the amino acids digestibility. In this experiment, it is appeared that keratinase improve the growth performance of broiler by increase average diary gain and feed conversion rate.
在线联系供应商
Other supplier products
Acid Protease | Acid Protease Protease catalyzes peptide bond and hydrolyzes protein into peptone, hydrazone, polypeptide and amino acid. Activity center of acid ... | |
Protease | Protease could hydrolyzes protein. It can efficiently hydrolyze the protein in feed materials and improve the utilization rate of protein. At the s... | |
Alpha Amylase | α- Amylase is an enzyme preparation abstracted from bacillus lincheniformis through deep-layer liquid fermentation, and is applied in such in... | |
Alpha Amylase | α- Amylase is an enzyme preparation abstracted from bacillus lincheniformis through deep-layer liquid fermentation, and is applied in such in... | |
Phytase | Phytase Phytase is a feed enzyme fermented with an excellent genetic engineering strain and produced by advanced post treatment. Phytase can break... |
Same products
丙酸钙 | 卖方: 新蒙德国际贸易有限公司 | 丙酸钙是一种新型食品添加剂,是世界卫生组织(WHO)和联合国粮农组织(FAO)批准使用的安全可靠的食品与饲料用防霉剂。丙酸钙对霉菌、酵母菌及细菌等具有广泛的抗菌作用。 1. 丙酸钙可用作食品及... | |
Factory Sale Top Quality Antibiotic Alternative Protect Intestinal Mucosa Glyceryl Tributyrate Tributyrin 60% Butyrate | 卖方: 烟台誉多生物科技有限公司 | ||
Animal allicin 25% feed powder fish aquatic probiotic chicken duck premix feed additive for cattle, sheep and pigs | 卖方: 烟台誉多生物科技有限公司 | Storage Type:used for animal feed Specification:25% Shelf Life:24month Manufacturer:Shandong ... | |
High Concentration Agriculture Bacillus Subtilis 10/20/50/100/200/500 Billion cfu/g | 卖方: 烟台誉多生物科技有限公司 | Type:bacillus subtilis Appearance:brown powder Sample:Available Storage:Dry Place Shelf life:... | |
Compound/multiple animal feed additive Mould inhibitor Calcium propionate >30%,Sodium diacetate, etc | 卖方: 烟台誉多生物科技有限公司 | Place of Origin:Shandong, China Type:Feed Grade Amino Acids, Feed Grade Proteins, Feed Grade Ant... |