Human Adrenomedullin ELISA kit

E01A0047 Human Adrenomedullin ELISA kit

Human Adrenomedullin ELISAkit is suitable for the detection of samples from human species. Adrenomedullin can also be called AM, ADM, ADM precursor, ADML.

Specifications of Human Adrenomedullin ADMELISAkit

Principle of the Assay

AM ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-AM antibody and an AM-HRP conjugate. The assay sample and buffer are incubated together with AM-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AM concentration since AM from samples and AM-HRP conjugate compete for the anti-AM antibody binding site. Since the number of sites is limited, as more sites are occupied by AM from the sample, fewer sites are left to bind AM-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AM concentration in each sample is interpolated from this standard curve.

Citations of Human Adrenomedullin ELISA kit

E01A0047 has been referenced in the below publications:

Analysis of the Secretome of Apoptotic Peripheral Blood Mononuclear Cells: Impact of Released Proteins and Exosomes for Tissue Regeneration.

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