BlueGene Biotech Bovine C Type Natriuretic Peptide ELISA kit
E11C0339 Bovine C Type Natriuretic PeptideELISA kit
Bovine C Type Natriuretic Peptide ELISAkit is suitable for the detection of samples from Bovine species. C Type Natriuretic Peptide can also be called NPPC; CNP2; Natriuretic peptide precursor C; natriuretic peptide C, CTNP.
Specifications of Bovine C Type Natriuretic Peptide ELISA kit
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Product Information |
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E11C0339 |
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Product Name |
Bovine C Type Natriuretic Peptide ELISA kit |
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Species |
Bovine |
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Product Size |
48 Tests / 96 Tests |
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Concentration |
50-1000 pg/mL |
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Sensitivity |
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Principal |
Competitive ELISA |
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Sample Volume |
100 ul |
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Sample Type |
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
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Assay Time |
90 minutes |
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Platform |
Microplate Reader |
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Conjugate |
HRP |
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Detection Method |
Colorimetric |
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Storage |
2-8°C |
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Kit Components |
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MATERIALS |
SPECIFICATION |
QUANTITY |
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MICROTITER PLATE |
96 wells |
stripwell |
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ENZYME CONJUGATE |
1 vial |
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STANDARD A (0.5mL) |
0 pg/mL |
1 vial |
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STANDARD B (0.5mL) |
50 pg/mL |
1 vial |
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STANDARD C (0.5mL) |
100 pg/mL |
1 vial |
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STANDARD D (0.5mL) |
250 pg/mL |
1 vial |
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STANDARD E (0.5mL) |
500 pg/mL |
1 vial |
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STANDARD F (0.5mL) |
1000 pg/mL |
1 vial |
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SUBSTRATE A |
6 mL |
1 vial |
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SUBSTRATE B |
6 mL |
1 vial |
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STOP SOLUTION |
6 mL |
1 vial |
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WASH SOLUTION (100 x) |
10 mL |
1 vial |
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BALANCE SOLUTION |
3 mL |
1 vial |
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Principle of the Assay |
CNP ELISAkit applies the competitive enzyme immunoassay technique utilizing an anti-CNP antibody and an CNP-HRP conjugate. The assay sample and buffer are incubated together with CNP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CNP concentration since CNP from samples and CNP-HRP conjugate compete for the anti-CNP antibody binding site. Since the number of sites is limited, as more sites are occupied by CNP from the sample, fewer sites are left to bind CNP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CNP peptideconcentration in each sample is interpolated from this standard curve.
Quality Control on Bovine C Type Natriuretic Peptide ELISA kit
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Coefficient of Variance |
Intra Variation% ＜10% |
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Inter Variation% ＜12% |
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Recovery |
90-101% |
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Linearity |
Diluent Ratio |
Range % |
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1:2 |
87-105 |
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1:4 |
84-108 |
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1:8 |
81-108 |
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Specificity/Cross-reactivity |
No significant cross-reactivity or interference between CNP and analogues was observed. |
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If you want to know more kinds of anp elisa, please visit our website.
As one bovine igg elisa kitsmanufacturer, we are committed to providing the kits with simple and easy operations but accurate and reliable quality to the researchers, and we have won a good reputation from customers all around the world. Many published papers have cited the usage of BlueGeneBiotech ELISA kits.
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