Human Angiopoietin 2 ELISA kit

NE01A0503 Human Angiopoietin 2 ELISA kit

Human angiotensin ii elisa kitis suitable for the detection of samples from the human species. Angiopoietin 2 can also be called as AGPT2, ANGPT2, ANG2, angiopoietin 2, and ANG-2.

Principle of the Assay

ANG2 angiotensin ii elisakit applies the solid phase sandwich enzyme immunoassay technique. The microplate has been pre-coated with antibody specific to ANG2. Standards or samples are then added to the microplate wells and ANG2 if present, will bind to the antibody pre-coated wells. Then a biotin-conjugateddetection antibody specific toANG2 is added to the wells to form a pre-coatedantibody-antigen-detection antibody Complex. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells, and then a TMB substrate solution is added to the wells to incubate. TMB substrate solution turns to blue after oxidating by HRP, and finally turns to yellow after adding the stop solution. Color develops in proportion to the amount of ANG2 bound in theinitial steps. The color is measured spectrophotometrically at a wavelength of 450nm. The concentration of ANG2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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