BlueGene Biotech Canine Collagen Type II Alpha 1 ELISA kit
E08C0323 Canine Collagen Type II Alpha 1 ELISA kit
Canine Collagen Type II Alpha 1 ELISA kit is suitable for the detection of samples from Canine species. Collagen Type II Alpha 1 can also be called COL2-A1, COL2A1, COL2 A1, SEDC, Chondrocalcin, COL2α1, COL2-α1, COL2 α1.
Specifications of Canine Collagen Type II Alpha 1 ELISA kit
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Product Information |
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E08C0323 |
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Product Name |
Canine Collagen Type II Alpha 1 ELISA kit |
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Species |
Canine |
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Product Size |
48 Tests / 96 Tests |
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Concentration |
10-250 ng/mL |
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Sensitivity |
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Principal |
Competitive ELISA |
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Sample Volume |
100 ul |
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Sample Type |
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
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Assay Time |
90 minutes |
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Platform |
Microplate Reader |
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Conjugate |
HRP |
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Detection Method |
Colorimetric |
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Storage |
2-8°C |
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Kit Components |
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MATERIALS |
SPECIFICATION |
QUANTITY |
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MICROTITER PLATE |
96 wells |
stripwell |
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ENZYME CONJUGATE |
1 vial |
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STANDARD A (0.5mL) |
0 ng/mL |
1 vial |
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STANDARD B (0.5mL) |
10 ng/mL |
1 vial |
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STANDARD C (0.5mL) |
25 ng/mL |
1 vial |
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STANDARD D (0.5mL) |
50 ng/mL |
1 vial |
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STANDARD E (0.5mL) |
100 ng/mL |
1 vial |
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STANDARD F (0.5mL) |
250 ng/mL |
1 vial |
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SUBSTRATE A |
6 mL |
1 vial |
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SUBSTRATE B |
6 mL |
1 vial |
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STOP SOLUTION |
6 mL |
1 vial |
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WASH SOLUTION (100 x) |
10 mL |
1 vial |
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BALANCE SOLUTION |
3 mL |
1 vial |
Principle of the Assay
COL2a1 ELISAkit applies the competitive enzyme immunoassay technique utilizing an anti-COL2a1 antibody and an COL2a1-HRP conjugate. The assay sample and buffer are incubated together with COL2a1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the COL2a1 concentration since COL2a1 from samples and COL2a1-HRP conjugate compete for the anti-COL2a1 antibody binding site. Since the number of sites is limited, as more sites are occupied by COL2a1 from the sample, fewer sites are left to bind COL2a1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The COL2a1 concentration in each sample is interpolated from this standard curve.
Quality Control on Canine Collagen Type II Alpha 1 ELISA kit
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Coefficient of Variance |
Intra Variation% ＜10% |
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Inter Variation% ＜12% |
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Recovery |
87-104% |
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Linearity |
Diluent Ratio |
Range % |
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1:02 |
87-105 |
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1:04 |
84-109 |
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1:08 |
81-108 |
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Specificity/Cross-reactivity |
No significant cross-reactivity or interference between COL2a1 and analogues was observed. |
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If you want to know more kinds of human igg elisa kits, please visit our website.
BluegeneBiotech has been committed to R&D of ELISA kits, making our ELISA kits more convenient for researchers to use on the premise of guaranteeing the quality, thus assisting scientific research for our customers. So far, we have received 1200 published papers from researchers with a total of about 500 kinds of ELISA Kits that have been cited. Bluegene ELISA kit has the advantages of high specificity, high sensitivity, and high stability.
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