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Fluorescence Filters

Hyperion Optics is a premium full-service photonics supplier. We specialize in optical design manufacturingspan across the spectrum. From UV, VIS, to IR, we fabricate custom optical components to system assemblies that meet unique and specialized applications.

Fluorescent filters are a class of filters classified by application type.

Fluorescence Filteris a fluorescence imaging filter for biomedical and life science instruments, the key components, the main role is in the biomedical fluorescence analysis system for the separation and selection of substances in the excitation and emission fluorescence Of the spectral characteristics of the band. It is usually required that the filter cut-off depth be greater than OD5 (optical density, OD = -lgT). The core requirements for filters used in fluorescence detection systems are high cut-off steepness, high transmittance, high positioning accuracy, high cut-off depth, and excellent environmental stability.

Fluorescent filter is a combination of three, three are excitation filters, emission filters and dichroic filters.

Excitation Filter (Exciter Filter, Excitation Filter, Excitation Filter): In the fluorescence microscopy filters, only the excitation wavelength of the filter can pass through the fluorescence. In the past, a short-pass filter was used, and now a band-pass filter is basically used. The housing is marked with arrows indicating the direction of propagation of the recommended light.

Emission Filter (Emitter Filter, Emitter): Select and transmit the fluorescence emitted by the sample, the other range of light cut-off. The wavelength of the emitted light is longer than the wavelength of the excitation light (closer to red). A band-pass filter or a long-wave-pass filter may be selected as the emission filter. The housing is marked with arrows indicating the direction of propagation of the recommended light.

Dichroic Mirror(Dichromic Beamsplitter, Dichromatic Beamsplitter): also known as dichroic mirrors or dichroic mirrors. And placed at an angle of 45 ° to the optical path of the microscope. This filter reflects one color of light (excitation light) and transmits another color of light (emitted light), the reflectivity of the excitation light is greater than 90% and the transmittance of the emitted light is greater than 90%. The impervious portion of the spectrum is reflected rather than absorbed. Filter in the transmitted light and reflected light color complement each other, and thus also known as dichroic filters.

Our fluorescence filters are designed for fluorescence imaging applications, with durability in mind and high-performance optical specifications in manufacturing. The filter substrate is made of quartz, which can achieve 1/10 lambda surface accuracy, while the thermal expansion coefficient of quartz is relatively small, can obtain higher image quality.

Fluorescence Bandpass Filter

CWL(nm)

FWHM(nm)

Transmission(%)

OpticalDensity

BlockingWavelength(nm)

TransmittedWavefrontError

Material

Diameter

340

29

80

≥6.0

362-580

1/4λ

UVFusedSilica

25

357

48

80

≥6.0

250-320

1/4λ

UVFusedSilica

25

370

40

80

≥6.0

250-340&394-800

1/4λ

UVFusedSilica

25

377

54

85

≥6.0

409-701

1/4λ

UVFusedSilica

25

386

27

90

≥6.0

300-360&510-700

1/4λ

UVFusedSilica

25

387

15

90

≥6.0

405-700

1/4λ

UVFusedSilica

25

390

45

90

≥6.0

1/4λ

UVFusedSilica

25

435

48

93

≥6.0

505-850

1/4λ

UVFusedSilica

25

438

28

93

≥6.0

250-415&460-600

1/4λ

UVFusedSilica

25

440

46

93

≥6.0

1/4λ

UVFusedSilica

25

447

65

93

≥6.0

490-800

1/4λ

UVFusedSilica

25

448

25

93

≥6.0

250-423&471-950

1/4λ

UVFusedSilica

25

452

53

93

≥6.0

370-410

1/4λ

UVFusedSilica

25

466

45

93

≥6.0

500-550

1/4λ

UVFusedSilica

25

472

35

93

≥6.0

250-442&498-640

1/4λ

UVFusedSilica

25

475

53

93

≥6.0

1/4λ

UVFusedSilica

25

486

36

93

≥6.0

250-455&510-660

1/4λ

UVFusedSilica

25

494

25

93

≥6.0

300-473&518-680

1/4λ

UVFusedSilica

25

500

29

93

≥6.0

250-473&525-670

1/4λ

UVFusedSilica

25

510

25

93

≥6.0

300-490&531-800

1/4λ

UVFusedSilica

25

512

30

93

≥6.0

300-480&540-950

1/4λ

UVFusedSilica

25

520

41

93

≥6.0

250-488&560-700

1/4λ

UVFusedSilica

25

525

18

93

≥6.0

403-455

1/4λ

UVFusedSilica

25

527

22

93

≥6.0

300-513&546-740

1/4λ

UVFusedSilica

25

530

62

93

≥6.0

400-485&569-750

1/4λ

UVFusedSilica

25

534

25

93

≥6.0

300-510&555-725

1/4λ

UVFusedSilica

25

535

48

93

≥6.0

250-497&588-1000

1/4λ

UVFusedSilica

25

540

56

93

≥6.0

450-500

1/4λ

UVFusedSilica

25

543

27

93

≥6.0

250-518&565-750

1/4λ

UVFusedSilica

25

549

21

93

≥6.0

300-530&568-850

1/4λ

UVFusedSilica

25

550

100

93

≥6.0

300-488&622-975

1/4λ

UVFusedSilica

25

560

32

93

≥6.0

1/4λ

UVFusedSilica

25

561

21

93

≥6.0

582-636

1/4λ

UVFusedSilica

25

572

33

93

≥6.0

250-547&602-925

1/4λ

UVFusedSilica

25

575

35

93

≥6.0

300-541&638-800

1/4λ

UVFusedSilica

25

578

22

93

≥6.0

300-558&598-850

1/4λ

UVFusedSilica

25

585

40

93

≥6.0

300-556&618-780

1/4λ

UVFusedSilica

25

592

49

93

≥6.0

440-555&631-725

1/4λ

UVFusedSilica

25

605

22

93

≥6.0

403-455

1/4λ

UVFusedSilica

25

615

26

93

≥6.0

300-592&638-850

1/4λ

UVFusedSilica

25

620

60

93

≥6.0

300-581&664-850

1/4λ

UVFusedSilica

25

628

38

93

≥6.0

250-598&659-925

1/4λ

UVFusedSilica

25

640

20

93

≥6.0

250-610&655-925

1/4λ

UVFusedSilica

25

648

20

93

≥6.0

300-621&677-925

1/4λ

UVFusedSilica

25

655

47

93

≥6.0

403-455

1/4λ

UVFusedSilica

25

661

26

93

≥6.0

300-638&684-850

1/4λ

UVFusedSilica

25

676

36

93

≥6.0

250-650

1/4λ

UVFusedSilica

25

692

47

93

≥6.0

520-662&733-900

1/4λ

UVFusedSilica

25

710

47

93

≥6.0

250-666&745-940

1/4λ

UVFusedSilica

25

716

47

93

≥6.0

535-682&758-900

1/4λ

UVFusedSilica

25

769

48

93

≥6.0

250-724&808-1025

1/4λ

UVFusedSilica

25

775

54

93

≥6.0

250-350,550-737,&837-975

1/4λ

UVFusedSilica

25

785

71

93

≥6.0

450-738&830-1050

1/4λ

UVFusedSilica

25

832

45

93

≥6.0

550-794&878-1075

1/4λ

UVFusedSilica

25



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