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• CAR-T Cell cell mediated Cytotoxicity Studies
• Phenotypic Charaterization of CAR-T cells
• Viability and Concentration assay for :
• 1. Quality control of original blood samples
• 2. PBMC isolation efficiency tests
• 3. Cultured status of incubated T-lymphocytes
• 4. Process Monitoring of modified CAR-T cells
• 5. Final Product released control.

Product Overview
TheCountstarS3 System combines the three fluorescence channels plus a bright field digital microscope, an image cytometer, and a cell counter in a single bench-top instrument. This application-driven, compact, automated cell imaging system provides an all-in-one solution for cell counting, cell viability and T/NK cell mediated cytotoxicity through the use of preconfigured biological applications (BioApps). They are designed to simplify routine cell lab tasks，which leaves you time to focus on the research!

Excitation light: 375nm, 480nm, 525nm
Emission filter：460nm, 535nm, 600LP
Key Benefits

• All in one design- Integrated Computer/Touch screen
• Automatic Detection 5 Samples in one single test sequence.
• Open platform with maximum 4 fluorescence Channel
• User Friendly Cell Analysis Protocols
• Unrivaled "Fixed focus" patent, no manual focusing.
• High Stability and Actuary
• Powerful and user-friendly Data Management
• GMP and 21 CFR part 11 ready
• FCS Express Software

Product feature
13 different fluorescent analysis combinations
User Friendly Cell Analysis Protocols
Trypan blue Protocols: Obtain cell count, viability and concentration estimations based on trypan blue staining using a disposable consumable.
AO/PI Viability Protocol: Run two fluorescence color assays in disposable consumable to determine the percentages of live, dead cells and concentration in the presents of debris and unwanted nonnucleated cell types including red blood cells.
Affinity of Antibody: This assay run as Green color assays in FITC binding antibody to determine the affinity of the biosimilar drug.
Surface Marker Assay: Quantify specific cell populations based on surface marker expression (CD45+ CD4+, CD8 + MSC, CD56+ NK cells, etc.)
Cell Cycle: Propidium iodide (PI) is a nuclear staining dye that is frequently applied in measuring cell cycle. The protocol determining cellular DNA content in cell cycle analysis
Cell Apoptosis: Cell Apoptosis assay is a type used for determining the apoptosis percentage of cells by Annexin V-FITC/7-ADD staining method.
GFP transfection: The green fluorescent protein (GFP) exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. This protocol can analyze counting and percentage of GFP.
Celling Killing: Run three fluorescence color assays in disposable consumable to determine the CAR T/NK-Mediated Cytotoxicity using Tracer and Viability Dyes

GMP and 21 CFR part 11 ready

FCS Express Software
Applications
T/NKcell mediatedcytotoxicity
By labeling the target tumor cells with CFSE or transfect with GFP. Hoechst 33342 is used for stain all cells (both T cells and tumor cells), alternatively, target tumor cells can be stained with CFSE, PI is used for stain the dead cells (both T cells and tumor cells). This staining strategy allows for the discriminate of different cells.

Cell Apoptosis
The different phases of apoptosis are segmented into an early, middle and late stage. Specified indicators can document the single stages of apoptosis. The Countstar® FL offers a visualized image-based solution and analyzes all of these steps with pre-loaded protocols in depth.
Cell CycleCountstar Rigel S6 enables users to get the result of a cell cycle quickly and accurately. Counstar Rigel S6 can analyze proportion of cell in different phase of the cell cycle.
Surface Maker Analysis
A lymphocyte subsets analysis is a typical experiment performed in cell related research fields and various diseases diagnosis. Countstar® Rigel S6 offers a faster and easier way to make immune cell typing more efficient. With visible cell images and powerful data analysis
Affinity of antibody
Detect affinity of antibody in cell level is an important indicator of monoclonal antibody detection using immunofluorescence method.The Countstar® Rigel S6 offers a rapid, direct and reliable evaluating method for the affinity of antibody detection in antibody drug screening

GFP TransfectionEfficiency
In cell and molecular biology, the GFP gene is frequently used as a reporter of expression. Currently, scientists are commonly using the fluorescent microscopes or flow cytometers to analyze the transfection efficiency of mammalian cells. But Flow cytometer requires a high-qualified and experienced operator. While Countstar Rigel S6 enables users to get the result of a transfection efficiency assay quickly and accurately.