.

BlueGene Biotech Chicken Total Adiponectin ELISA kit

E12A0125 Chicken Total Adiponectin ELISA kit

Chicken Total Adiponectin ELISA kit (a knid of chicken elisakits )is suitable for the detection of samples from chicken species. Total Adiponectin can also be called 30 kDa adipocyte complement related protein, 30 kDa adipocyte complement-related protein, ACDC, ACRP 30, ACRP30, ADIPO, Adipocyte, Adipocyte C1q and collagen domain containing protein, Adipocyte complement related 30 kDa protein, Adipocyte complement related protein of 30 kDa, Adipocyte complement-related 30 kDa protein, Adiponectin, AdipoQ, Adipose most abundant gene transcript 1, Adipose most abundant gene transcript 1 protein, Adipose specific collagen like factor, ADIPQTL1, ADPN, APM 1, apM-1, ApM1, C1q and collagen domain-containing protein, GBP 28, GBP28, Gelatin binding protein, Gelatin binding protein 28, Gelatin-binding protein.

Product Information

E12A0125

Product Name

Chicken Total Adiponectin ELISA kit

Species

Chicken

Product Size

48 Tests / 96 Tests

Concentration

Sensitivity

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C

Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

1 vial

STANDARD C (0.5mL)

1 vial

STANDARD D (0.5mL)

1 vial

STANDARD E (0.5mL)

10 ng/mL

1 vial

STANDARD F (0.5mL)

25 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial

Principle of the Assay

ADPN ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-ADPN antibody and an ADPN-HRP conjugate. The assay sample and buffer are incubated together with ADPN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADPN concentration since ADPN from samples and ADPN-HRP conjugate compete for the anti-ADPN antibody binding site. Since the number of sites is limited, as more sites are occupied by ADPN from the sample, fewer sites are left to bind ADPN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADPN concentration in each sample is interpolated from this standard curve.

Quality Control on Chicken Total Adiponectin ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% 12%

Recovery

87-116.79%

Linearity

Diluent Ratio

Range %

1:2

96-116

1:4

92-105

1:8

88 -103

Specificity/Cross-reactivity

No significant cross-reactivity or interference between ADPN and analogues was observed.

If you want to know more kinds of research reagents, please visit our website.

Bluegene Elisa kitsupplier Shanghai BlueGene Biotech is a company with research, production, and sale in one, our product's range includes Enzyme-Linked Immunosorbent Assay (ELISA) kits and registered trademark "cgbio®" ELISA Kits, proteins, antibodies, plasmids, and other reagents, such as His-tag Protein Purification kit. We offer thousands of ELISA kits for researchers worldwide since 2008.



在线联系供应商

至: Bluegene Biotech
Your E-mail:
消息正文:


Send to other suppliers

Other supplier products

BlueGene Biotech Canine Endothelin 1 ELISA kit
BlueGene Biotech Canine Endothelin 1 ELISA kit E08E0040 Canine Endothelin 1 ELISAkit Canine Endothelin 1 ELISA kitis suitable for the detection of samples from Canine species. Endothelin 1 ca...
BlueGene Biotech Mouse Angiotensin (1-7) ELISA kit
BlueGene Biotech Mouse Angiotensin (1-7) ELISA kit E03A0206 Mouse Angiotensin (1-7) ELISA kit Mouse ang 1 7 elisa kitis suitable for the detection of samples from mouse species. Angiotensin (1-7)...
Host Cell Protein Detection Kits
Host Cell Protein Detection Kits CHO Host Cell Protein ELISA Kit, G1CHO Host Cell Protein ELISA Kit, G2E. coli Host Cell Protein ELISA Kit, G1HEK 293 Host Cell Protein ELISA Kit, G...
BlueGene Biotech Canine Collagen Type II Alpha 1 ELISA kit
BlueGene Biotech Canine Collagen Type II Alpha 1 ELISA kit E08C0323 Canine Collagen Type II Alpha 1 ELISA kit Canine Collagen Type II Alpha 1 ELISA kit is suitable for the detection of samples from Canin...
BlueGene Biotech Canine Brain Derived Neurotrophic Factor ELISA kit
BlueGene Biotech Canine Brain Derived Neurotrophic Factor ELISA kit E08B0029 Canine Brain Derived Neurotrophic Factor ELISA kit Canine Brain Derived Neurotrophic Factor BDNFELISA kitis suitable for the detection ...
供应产品

Same products

BlueGene Biotech Sheep Cluster of Differentiation 4 ELISA kit
BlueGene Biotech Sheep Cluster of Differentiation 4 ELISA kit 卖方: Bluegene Biotech E14C0004 Sheep Cluster of Differentiation 4 ELISA kit Sheep Cluster of Differentiation 4 ELISA...
BlueGene Biotech Sheep Anti Interleukin 10 ELISA kit
BlueGene Biotech Sheep Anti Interleukin 10 ELISA kit 卖方: Bluegene Biotech Specifications of Sheep Anti Interleukin 10 ELISAkit Product Information E1...
BlueGene Biotech Chicken Total Adiponectin ELISA kit
BlueGene Biotech Chicken Total Adiponectin ELISA kit 卖方: Bluegene Biotech E12A0125 Chicken Total Adiponectin ELISA kit Chicken Total Adiponectin ELISA kit (a knid of ch...
BlueGene Biotech Bovine Decorin ELISA kit
BlueGene Biotech Bovine Decorin ELISA kit 卖方: Bluegene Biotech E11D0145 Bovine Decorin ELISA kit Bovine Decorin ELISAkit is suitable for the detection of sam...
BlueGene Biotech Bovine C Type Natriuretic Peptide ELISA kit
BlueGene Biotech Bovine C Type Natriuretic Peptide ELISA kit 卖方: Bluegene Biotech E11C0339 Bovine C Type Natriuretic PeptideELISA kit Bovine C Type Natriuretic Peptide ELISAkit...