Colony Counter
Liquid Chromogenic Culture Medium
Coliforms and E. coli liquid chromogenic medium
Performance Characteristics of Liquid Chromogenic Culture Media
Product Details of Liquid Chromogenic Culture Media
|
Name |
Model |
Application |
Capacity |
Packing |
Validity Period |
|
Liquid chromogenic culture medium |
XM-30 |
For 1ml sample inspection |
10ml/cup |
128 cups/carton |
1 year |
|
Liquid chromogenic culture medium |
XM-31 |
For 10ml sample inspection |
10ml/cup |
128 cups/carton |
|
|
Liquid chromogenic culture medium |
XM-32 |
For 5ml sample inspection |
5ml/cup |
128 cups/carton |
|
|
XM powder medium |
XM0001 |
For preparing medium |
20 bags/carton |
3 years |
|
|
MT opener |
OS-01 |
For MT container opening |
/ |
1pc |
/ |
Usage Method of Broth Media Microbiology
- microbiology sample testdiluent: use a pipette to inject the sample dilution into the cup
XM-30: 1ml, XM-31: 10ml, XM-32: 5ml
※XM powder medium usage: Dissolve 500ml of pure water per bag, inject 10ml into the test tube, autoclave, then inject 1ml sample, and incubate at 36±1°C for 24 hours. The identification method is the same.
Coloring Principle
Coliforms positive
There is a special β-galactosidase only exit in the coliforms. It can be used as an indicator enzyme for coliform bacteria, which catalyzes the hydrolysis of the substrate X-GAL to form 5,5-dibromo -4,4-dichloro indigo, appearing light blue ~ purple-blue.
β--glucuronidase can be used as an indicator enzyme for Escherichia coli; because β-glucuronidase can be observed in Escherichia coli, Salmonella and Shigella in intestinal bacteria, and 95% of E. coli contains β-glucuronidase. It catalyzes the hydrolysis of MUG to form 4-methylumbelliferon, which exhibits fluorescence at 366 nm ultraviolet light and is visually discernible under indoor light intensity.
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