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Alkaline Protease

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CAS :9014-01-1 EC 3.4.21.14

Product Properties: The light yellow solid powder of light yellow liquid.


Overview: The alkaline protease is bred by the bacterial protoplast mutagenesis of Bacillus licheniformis 2709, hydrolase by deep fermentation, extraction and purification of a protein from its main enzyme composition for the Bacillus licheniformis protease is a serine-type endo proteases, it hydrolyzed protein peptide chain generates a peptide or amino acids, with a strong ability to break down proteins, are widely used in food, medicine, brewing, washing, silk, leather and other industries.

Product Features:

1.An alkaline protease production process using micro filtration, ultrafiltration membrane separation, spray drying and vacuum freeze drying, advanced technology, product quality to meet the quality standards of food grade and pharmaceutical grade, activity in the range of 200,000 to 1.5 million u / g can be formulated in accordance with the requirements of the customers.

2, the alkaline protease is an alkaline protein, the catalytic site serine, with excellent performance under high temperature and moderate alkalinity.

Application areas:

1, the alkaline protease is a non-toxic, no side effects of the protein, belongs to the serineprotease cutting within the application in the food industry can be hydrolyzed protein peptide chains to generate peptides or amino acids, the formation of the protein hydrolyzate has a unique flavor.

2.Alkaline protease successfully used in the detergent enzyme industry, which added to the ordinary detergent, concentrated laundry detergent and liquid detergent, both for the family laundry, can also be used for industrial laundry, and can effectively remove blood stains, eggs protein, dairy products, juices, juice stains, in addition to cleaning biochemical instruments as the medical reagent enzyme .

3, in the field of biotechnology, alkaline protease used as a tool enzyme protein in the purification of nucleic acids (including nucleic acid enzymes) to remove, no degradation of DNA, to avoid damage to the DNA integrity.

Vitality:

Enzyme activity is defined: Ultraviolet Spectrophotometry: determination of the conditions (40 ± 0.2 ° C; PH value 10.5) casein hydrolyzate per minute release of tri-chloroacetic acid soluble matter in the 275nm wavelength absorbance with the absorbance of 1 mg of tyrosine equivalent, the amount of enzyme required is a dynamic unit, u / g. Product standards: The product complies with US Food Chemicals Codex (FCC), the Ministry of Light Industry standard (QB/T1803-93), food grade enzyme specifications

Conditions of Use:
For the enzymatic aspects, the substrate concentration of 10-25%, temperature 50-60 ° C, pH values 8.5-10.5, the reaction time of 3-6 hours (depending on requirements can be long or short), add the enzyme 0.1-0.2% (the weight of the aqueous solution of dollars). Two other industrial and medical fields, with reference to relevant industry standards.

Storage: Recommendations in a cool, dry environment protected from light, storage temperature: below zero; Storage too long or adverse storage conditions, make the activity was significantly lower; such as temperature and humidity is too high, you need to use an appropriate increase in usage.

Security: Is a natural enzyme preparation of alkaline protease, protein food as edible food enzyme preparations contain protein foods are generally beneficial and harmless to the human body. For some sensitive populations, such as the direct intake of a high concentration of enzyme powder or mist may cause irritation, prolonged contact may irritate the skin, eyes and mucosal tissue. Recommended to wear masks, goggles and other protective equipment during operation, the surplus or spill out of the enzyme powder and timely treatment, gently sweep back to the container for a large number of enzyme powder spilled a small amount is sucked by vacuum or water soaked clean up.

The alkaline protease is a biologically active substance, susceptible to inhibit the damaging effects of heavy metal ions (Fe3 + and Cu2 +, Hg +, Pb +, etc.) and the oxidant, and should avoid any contact with the process ofstoring or using.

Packing: Aluminum bags, 1kg x 10 packs / box; 1kg x 20 bags / box Plastic bottles, 0.5kg / bottle x 20kg / box



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