Large Naïve VHH Library
Large Naïve VHH Library
VHHare single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin.The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.
Phage display and yeast display are the most powerful and widely used laboratory technique for the study of protein-protein, protein-peptide and protein-DNA interactions. They are mainly based on displaying the interest protein (peptides, antibodies, scaffolds or others) on the surface of employing phage or yeast and then be used to interrogate the constructed libraries containing millions or even billions of displayed phages or yeasts.
Phage display and yeast display are the most powerful and widely used laboratory technique for the study of protein-protein, protein-peptide and protein-DNA interactions. They are mainly based on displaying the interest protein (peptides, antibodies, scaffolds or others) on the surface of employing phage or yeast and then be used to interrogate the constructed libraries containing millions or even billions of displayed phages or yeasts.
Here we provide large naïve VHH libraries using PBMCs and spleens from more than 50 healthy nonimmunized Alpacas/Llamas/Camels separately.
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