Human Adenosine Triphosphate ELISA kit

• 

E01A0038 Human Adenosine Triphosphate ELISA kit

Human Adenosine Triphosphate ATP ELISAkit is suitable for the detection of samples from human species. Adenosine Triphosphate can also be called Adenosine-5'-Triphosphate, ATP.

Specifications of Human Adenosine Triphosphate ATPELISA kit

Principle of the Assay

ATP ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-ATP antibody and an ATP-HRP conjugate. The assay sample and buffer are incubated together with ATP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ATP concentration since ATP from samples and ATP-HRP conjugate compete for the anti-ATP antibody binding site. Since the number of sites is limited, as more sites are occupied by ATP from the sample, fewer sites are left to bind ATP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ATP concentration in each sample is interpolated from this standard curve.

Quality Control on Human Adenosine Triphosphate ELISA kit

Citations of Human Adenosine Triphosphate ELISA kit

E01A0038 has been referenced in the below publications:

The research of the metabolism and in vivo functional changes of erythrocyte stored at -65℃.

Elisa kit supplier Shanghai BlueGene Biotechis a company with research, production, and sale in one, our product's range includes Enzyme-Linked Immunosorbent Assay (ELISA) kits and registered trademark "cgbio®" ELISA Kits, proteins, antibodies, plasmids, and other reagents, such as His-tag Protein Purification kit.

If you want to know more kinds of human insulin elisa kit, please visit our website.

Другие товары поставщика

	Expression Plasmids	cre expression plasmidare a kind of double-stranded, closed-loop DNA with a size range from 1kb to more than 200kb. All kinds of antibody expressio...
	Human ELISA Kits	Our more than 8000 human visfatin elisa kitare intended to advance your research to study human disease covering several research areas (like cance...
	BlueGene Biotech Porcine Soluble Cluster Of Differentiation 40 Ligand ELISA kit	E07C0209 Porcine Soluble Cluster Of Differentiation 40 Ligand ELISA kit Porcine soluble Cluster of differentiation 40 ligand ELISA kit is suitab...
	BlueGene Biotech	Bovine Carnitine Palmitoyltransferase I ELISA kit Metabolism E11C0055 Bovine Carnitine Palmitoyltransferase IELISA kit Bovine Carnitine Palmit...
	BlueGene Biotech Canine β Cell Leukemia/Lymphoma 2 ELISA kit	E08B0033 Canine β Cell Leukemia/Lymphoma 2 ELISA kit Canine β Cell Leukemia/Lymphoma 2 ELISA kit is suitable for the detection of samp...

Похожие товары

	Peptide Technology Cooperation & Transfer	Продавец: PeptiOrigin Biotechnology Co., Ltd.	Peptide Technology Cooperation & Transfer Driving Innovation Through Strategic Partnerships ...
	Recombinant Human Fibroblast Growth Factor-basic	Продавец: Hangzhou NeuroPeptide Biological Science and Technology Incorporation, Ltd	Physical Appearance:Freeze-dried powder Formulation:5mMPB(pH7.4) before freeze-drying TheorypI:...
	Recombinant Staphylococcal Protein A	Продавец: Hangzhou NeuroPeptide Biological Science and Technology Incorporation, Ltd	TheorypI:5.10 Resources:Escherichia coli(E. coli) Purity:≥95% Storage condition:-20℃ Stora...
	NuPerley Core 700	Продавец: Hangzhou NeuroPeptide Biological Science and Technology Incorporation, Ltd	NuPerley Core 700 Matrix：Rigid agarose microsphere Ligand：n-octylamine Particle size, d50：~90 ...
	Q NUPharose Fast Flow	Продавец: Hangzhou NeuroPeptide Biological Science and Technology Incorporation, Ltd	Matrix：6% Cross-linked agarose Ligand：Quaternary ammonium salt, -CH2CH(OH)CH2N(CH3)3+ Particle ...