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Human Adenosine Triphosphate ELISA kit

E01A0038 Human Adenosine Triphosphate ELISA kit

Human Adenosine Triphosphate ATP ELISAkit is suitable for the detection of samples from human species. Adenosine Triphosphate can also be called Adenosine-5'-Triphosphate, ATP.

Specifications of Human Adenosine Triphosphate ATPELISA kit

Product Information

E01A0038

Product Name

Human Adenosine Triphosphate ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

50-1000 ng/mL

Sensitivity

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C

Principle of the Assay

ATP ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-ATP antibody and an ATP-HRP conjugate. The assay sample and buffer are incubated together with ATP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ATP concentration since ATP from samples and ATP-HRP conjugate compete for the anti-ATP antibody binding site. Since the number of sites is limited, as more sites are occupied by ATP from the sample, fewer sites are left to bind ATP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ATP concentration in each sample is interpolated from this standard curve.

Quality Control on Human Adenosine Triphosphate ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

94-106%

Linearity

Diluent Ratio

Range %

1:02

93-101

1:04

90-107

1:08

92-105

Specificity/Cross-reactivity

No significant cross-reactivity or interference between ATP and analogues was observed.

Citations of Human Adenosine Triphosphate ELISA kit

E01A0038 has been referenced in the below publications:

The research of the metabolism and in vivo functional changes of erythrocyte stored at -65℃.

Elisa kit supplier Shanghai BlueGene Biotechis a company with research, production, and sale in one, our product's range includes Enzyme-Linked Immunosorbent Assay (ELISA) kits and registered trademark "cgbio®" ELISA Kits, proteins, antibodies, plasmids, and other reagents, such as His-tag Protein Purification kit.

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